5'-Nucleotidase Active Protein | Nt5e active protein

5'-Nucleotidase, Recombinant, Mouse (CD73, Ecto-5'nucleotidase, 5-NT)

Cat. Num. AAA637721

• Gene Names: Nt5e; Nt5; CD73
• Purity: >90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Synonyms: 5'-Nucleotidase; Recombinant; Mouse (CD73; Ecto-5'nucleotidase; 5-NT); Nt5e active protein

• Purity/Purification: >90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a liquid in 20mM Tris, 0.12M sodium chloride, 4mM CaCl2, 20% glycerol, pH 7.5.
• Concentration: 0.44mg/ml (varies by lot)

• Immunogen: CHO-derived recombinant mouse 5'-Nucleotidase/CD73.
• Specific Activity: >10,000 pmoles/min/ug.
• Biological Activity: Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit.
• Endotoxin: <1Eu/mg
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Nt5e active protein

CD73 (Ecto-5'nucleotidase, 5 '-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.

Recombinant mouse 5'-Nucleotidase/CD73 ectodomain (rmCD73) encoded by the NT5E gene was expressed with a C-terminal 6X His tag in a Chinese Hamster Ovary cell line. CD73, an ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5'-nucleotidase activity of CD73 converts extracellular nucleoside-5'-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).
The purified, secreted rmCD73 has a N-terminal sequence of W29ELTILHTND. The 527 amino acid rmCD73 predicts a molecular mass of 59kD and migrates as a 70kD band in SDS-PAGE under reducing conditions.

Product Categories/Family for Nt5e active protein

Molecular Biology; MB-Enzymes; Nucleotidase

NCBI and Uniprot Product Information

• NCBI GI #: 148747417
• NCBI GeneID: 58813
• NCBI Accession #: NP_067587.2
• NCBI GenBank Nucleotide #: NM_021576.2
• UniProt Accession #: P21588
• Molecular Weight: 70kD
• NCBI Official Full Name: 5'-nucleotidase
• NCBI Official Synonym Full Names: 5' nucleotidase, ecto
• NCBI Official Symbol: Nt5e
• NCBI Official Synonym Symbols: Nt5; CD73
• NCBI Protein Information: 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• Protein Family: 5'-nucleotidase
• UniProt Gene Name: Nt5e
• UniProt Synonym Gene Names: Nt5; Nte; 5'-NT
• UniProt Entry Name: 5NTD_RAT

NCBI Description

may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Nucleotide Metabolism - pyrimidine; Phosphatase (non-protein); EC 3.1.3.5; Membrane protein, integral; Membrane protein, GPI anchor

Cellular Component: cell surface; membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: 5'-nucleotidase activity; ferrous iron binding; metal ion binding; nucleotide binding

Biological Process: dephosphorylation; adenosine biosynthetic process; negative regulation of inflammatory response; brain development; positive regulation of lipid biosynthetic process; purine nucleotide biosynthetic process; adenosine metabolic process; AMP catabolic process; response to aluminum ion