Human Apoptosis-associated speck-like protein containing a CARD ELISA Kit | PYCARD elisa kit

Human Apoptosis-associated speck-like protein containing a CARD ELISA Kit

Cat. Num. AAA2887570

• Gene Names: PYCARD; ASC; TMS; TMS1; CARD5; TMS-1
• Reactivity: Human
• Synonyms: Apoptosis-associated speck-like protein containing a CARD; Human Apoptosis-associated speck-like protein containing a CARD ELISA Kit; hASC; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; PYCARD elisa kit

• Reactivity: Human
• Sequence Length: 195

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/mL
• Sensitivity: 0.078 ng/mL
• Intra-Assay CV: <=4.9%
• Inter-Assay CV: <=7.3%
• Recovery: 90%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PYCARD elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10835256
• NCBI GeneID: 29108
• NCBI Accession #: NP_037390.2
• NCBI GenBank Nucleotide #: NM_013258.4
• UniProt Accession #: Q9ULZ3; Q96D12; Q9BSZ5; Q9HBD0; Q9NXJ8
• Molecular Weight: 15,030 Da
• NCBI Official Full Name: apoptosis-associated speck-like protein containing a CARD isoform a
• NCBI Official Synonym Full Names: PYD and CARD domain containing
• NCBI Official Symbol: PYCARD
• NCBI Official Synonym Symbols: ASC; TMS; TMS1; CARD5; TMS-1
• NCBI Protein Information: apoptosis-associated speck-like protein containing a CARD
• UniProt Protein Name: Apoptosis-associated speck-like protein containing a CARD
• Protein Family: Apoptosis-associated speck-like protein
• UniProt Gene Name: PYCARD
• UniProt Synonym Gene Names: ASC; CARD5; TMS1; hASC
• UniProt Entry Name: ASC_HUMAN

NCBI Description

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PYCARD: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; IkappaB kinase complex; mitochondrion; nucleolus; nucleus

Molecular Function: caspase activator activity; enzyme binding; identical protein binding; interleukin-6 receptor binding; myosin I binding; protease binding; protein binding; protein homodimerization activity; Pyrin domain binding; tropomyosin binding

Biological Process: activation of innate immune response; activation of NF-kappaB transcription factor; apoptosis; caspase activation; defense response to Gram-negative bacterium; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; inflammatory response; inhibition of NF-kappaB transcription factor; innate immune response; myeloid dendritic cell activation; myeloid dendritic cell activation during immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interferon-beta production; positive regulation of actin filament polymerization; positive regulation of activated T cell proliferation; positive regulation of adaptive immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of interferon-gamma production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of phagocytosis; positive regulation of T cell activation; positive regulation of transcription factor activity; positive regulation of tumor necrosis factor production; regulation of protein stability; signal transduction; stimulatory C-type lectin receptor signaling pathway; tumor necrosis factor-mediated signaling pathway