Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Goat PIR51/RAD51AP1 Polyclonal Antibody | anti-RAD51AP1 antibody
Goat anti-PIR51/RAD51AP1 Antibody
Gene Names
RAD51AP1; PIR51
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Cow
Applications
ELISA, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PIR51/RAD51AP1; Polyclonal Antibody; Goat anti-PIR51/RAD51AP1 Antibody; RAD51AP1; PIR51; RAD51-interacting protein; RAD51 associated protein 1; RAD51AP1 antibody; PIR51 antibody; RAD51-interacting protein antibody; RAD51 associated protein 1 antibody; PIR51 / RAD51AP1; anti-RAD51AP1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
ARVKPLHPNATST
Sequence Length
352
Applicable Applications for anti-RAD51AP1 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatocytes and spermatids. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave an approx 25kDa band in Human Testis lysates at 0.2ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx.39kDda according to NP_006470.
Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatocytes and spermatids. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave an approx 25kDa band in Human Testis lysates at 0.2ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx.39kDda according to NP_006470.
Immunogen
Peptide with sequence C-ARVKPLHPNATST, from the C Terminus of the protein sequence according to NP_001124334.1; NP_006470.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,142 Da
NCBI Official Full Name
RAD51-associated protein 1 isoform a
NCBI Official Synonym Full Names
RAD51 associated protein 1
NCBI Official Symbol
RAD51AP1
NCBI Official Synonym Symbols
PIR51
NCBI Protein Information
RAD51-associated protein 1
UniProt Protein Name
RAD51-associated protein 1
Protein Family
UniProt Gene Name
RAD51AP1
UniProt Entry Name
R51A1_HUMAN
Uniprot Description
RAD51AP1: May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA. 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 12p13.2-p13.1
Cellular Component: nucleoplasm; nucleus
Molecular Function: double-stranded DNA binding; protein binding; RNA binding; single-stranded DNA binding
Biological Process: DNA repair; DNA synthesis during DNA repair; double-strand break repair via homologous recombination; strand displacement
Research Articles on RAD51AP1
Similar Products
Product Notes
The RAD51AP1 rad51ap1 (Catalog #AAA420708) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PIR51/RAD51AP1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's PIR51/RAD51AP1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatocytes and spermatids. Recommended concentration, 4-6ug/ml. Western Blot: Preliminary experiments gave an approx 25kDa band in Human Testis lysates at 0.2ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx.39kDda according to NP_006470. Researchers should empirically determine the suitability of the RAD51AP1 rad51ap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ARVKPLHPNA TST. It is sometimes possible for the material contained within the vial of "PIR51/RAD51AP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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