Typical Testing Data/Standard Curve (for reference only)
MTT Cell Proliferation and Cytotoxicity Accessory Reagent
MTT Cell Proliferation and Cytotoxicity Assay Kit
Synonyms
MTT Cell Proliferation and Cytotoxicity; MTT Cell Proliferation and Cytotoxicity Assay Kit; MTT Cell Proliferation and Cytotoxicity accessory reagent
Samples
Tissue And Cell Samples
Preparation and Storage
MTT staining solution should be stored at -20 degree C in dark; Formazan diluent solution should be stored at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MTT Cell Proliferation and Cytotoxicity accessory reagent
Principle of the Assay: DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.
Product Categories/Family for MTT Cell Proliferation and Cytotoxicity accessory reagent
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Product Notes
The MTT Cell Proliferation and Cytotoxicity (Catalog #AAA176419) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MTT Cell Proliferation and Cytotoxicity, Accessory Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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