Pyruvate Kinase (PK) Assay Kit | PK assay kit
Pyruvate Kinase (PK) Colorimetric Assay Kit
Synonyms
Pyruvate Kinase (PK); Pyruvate Kinase (PK) Colorimetric Assay Kit; PK assay kit
Kit Components
Extraction Buffer
Assay Buffer
Substrate Mix
LDH
Assay Buffer
Substrate Mix
LDH
Features & Benefits
Determination of Pyruvate kinase (PK) activity in cells, bacteria, serum (plasma), tissue/cell lysates and other biological fluids.
Kit components and procedures are simple.
Sample preparation, experimental steps and result calculation are more detailed and accurate.
Long shelf life.
Kit components and procedures are simple.
Sample preparation, experimental steps and result calculation are more detailed and accurate.
Long shelf life.
Usage Notes
Assay kit is intended for research use only. Not for use in test procedures. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Do not mix or substitute kit reagents or materials from other lots or vendors. Kits are QC tested as a set of components and performance cannot be guaranteed if utilized separately or substituted.
Avoid foaming or bubbles when mixing or reconstituting components.
Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
Ensure all reagents and equipment are at the appropriate temperature before assay.
96-well UV microplate is needed
Sample preparation and other processes need to be performed on ice, and the enzyme activity must be measured on the same day and avoid repeated freezing and thawing to prevent enzyme inactivation.
LDH must be placed on ice during the measurement process. The reaction temperature has an influence on the results, so please keep it at 25 degree C(general species) or 37 degree C(mammal).
Do not mix or substitute kit reagents or materials from other lots or vendors. Kits are QC tested as a set of components and performance cannot be guaranteed if utilized separately or substituted.
Avoid foaming or bubbles when mixing or reconstituting components.
Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
Ensure all reagents and equipment are at the appropriate temperature before assay.
96-well UV microplate is needed
Sample preparation and other processes need to be performed on ice, and the enzyme activity must be measured on the same day and avoid repeated freezing and thawing to prevent enzyme inactivation.
LDH must be placed on ice during the measurement process. The reaction temperature has an influence on the results, so please keep it at 25 degree C(general species) or 37 degree C(mammal).
Preparation and Storage
Storage at -20 degree C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping: Gel pack with blue ice.
Shipping: Gel pack with blue ice.
Related Product Information for PK assay kit
Pyruvate Kinase (PK) Colorimetric Assay Kit is designed for detecting the Pyruvate Kinase activity in the sample.
Background: PK (EC 2.7.1.40) is widely present in animals, plants, microorganisms and cultured cells, and catalyzes the last step reaction in the glycolysis process. It is one of the main rate-limiting enzymes in the glycolysis process and the key to ATP production. It is important to measure PK activity.
Background: PK (EC 2.7.1.40) is widely present in animals, plants, microorganisms and cultured cells, and catalyzes the last step reaction in the glycolysis process. It is one of the main rate-limiting enzymes in the glycolysis process and the key to ATP production. It is important to measure PK activity.
Product Categories/Family for PK assay kit
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Product Notes
The PK (Catalog #AAA9719073) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Pyruvate Kinase (PK), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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