Typical Testing Data/Standard Curve (for reference only)
(Fig. Standard Curve of acetyl coenzyme A assay. The x-axis is OD340 and the y-axis is acetyl coenzyme A concentration (nmol/mL).)
Acetyl Coenzyme A (Acetyl-CoA) Assay Kit | Acetyl-CoA assay kit
Acetyl Coenzyme A (Acetyl-CoA) Colorimetric Assay Kit
Synonyms
Acetyl Coenzyme A (Acetyl-CoA); Acetyl Coenzyme A (Acetyl-CoA) Colorimetric Assay Kit; Acetyl-CoA assay kit
Tag
Acetyl-CoA
Features & Benefits
simple, sensitive, rapid colorimetric Acetyl-CoA detection method.
compatible to various biological samples, especially animal or plant tissues or cell cultures (adherent or suspension).
Provides detailed sample preparation and results calculation methods.
Acetyl Coenzyme A: Acetyl-CoA Assay Kit provides a simple, sensitive, rapid colorimetric Acetyl-CoA detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures: adherent or suspension The detection principle is that malate dehydrogenase can catalyze malic acid and NAD to produce oxaloacetic acid and NADH. citrate synthase can catalyze Acetyl-CoA and oxaloacetic acid to produce citric acid and CoA. Based on the coupling reaction of malate dehydrogenase and citrate synthase, Acetyl-CoA content is is proportional to the generating rate of NADH. NADH has a special absorption peak at 340nm, and the calculation of the absorbance value of 340nm can be obtained the content of Acetyl-CoA.
compatible to various biological samples, especially animal or plant tissues or cell cultures (adherent or suspension).
Provides detailed sample preparation and results calculation methods.
Acetyl Coenzyme A: Acetyl-CoA Assay Kit provides a simple, sensitive, rapid colorimetric Acetyl-CoA detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures: adherent or suspension The detection principle is that malate dehydrogenase can catalyze malic acid and NAD to produce oxaloacetic acid and NADH. citrate synthase can catalyze Acetyl-CoA and oxaloacetic acid to produce citric acid and CoA. Based on the coupling reaction of malate dehydrogenase and citrate synthase, Acetyl-CoA content is is proportional to the generating rate of NADH. NADH has a special absorption peak at 340nm, and the calculation of the absorbance value of 340nm can be obtained the content of Acetyl-CoA.
Kit Components
Extraction Buffer
ReagentI
Reagent II
Reagent III
Reagent IV
Standard
ReagentI
Reagent II
Reagent III
Reagent IV
Standard
Usage Notes
Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
All samples and reagents should be on ice to avoid denaturation and deactivation.
Please to extract Acetyl-CoA from fresh samples in order to ensure the enzyme activity.
Please reduce the tissues weight if the OD values is too high,or reduce the volume of Extraction Buffer if the OD values is too low.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
All samples and reagents should be on ice to avoid denaturation and deactivation.
Please to extract Acetyl-CoA from fresh samples in order to ensure the enzyme activity.
Please reduce the tissues weight if the OD values is too high,or reduce the volume of Extraction Buffer if the OD values is too low.
Preparation and Storage
The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Typical Testing Data/Standard Curve (for reference only)
(Fig. Standard Curve of acetyl coenzyme A assay. The x-axis is OD340 and the y-axis is acetyl coenzyme A concentration (nmol/mL).)
Typical Testing Data/Standard Curve (for reference only)
(Fig. Standard Curve of acetyl coenzyme A assay. The x-axis is OD340 and the y-axis is acetyl coenzyme A concentration (nmol/mL).)
Related Product Information for Acetyl-CoA assay kit
Description: Acetyl Coenzyme A (Acetyl-CoA) Assay Kit provides a simple, sensitive, rapid colorimetric Acetyl-CoA detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension)
Background: Acetyl-CoA is widely present in animals, plants, microorganisms and cultured cells. It is an important intermediate metabolite produced during the metabolism of biometrics. In vivo energy substance metabolism is a hinging material. The three major nutrients --glucose, lipid, protein which could be via acetyl coenzyme A to form a common metabolic pathway - tricarboxylic acid circulation and phosphorylation. Through this path, Acetyl-CoA thoroughly could be oxidize carbon dioxide and water, and release energy for ATP synthesis. Further, Acetyl-CoA a is a precursor material while synthetic fatty acid, ketone body, and Cholesterol and its derivatives.
Background: Acetyl-CoA is widely present in animals, plants, microorganisms and cultured cells. It is an important intermediate metabolite produced during the metabolism of biometrics. In vivo energy substance metabolism is a hinging material. The three major nutrients --glucose, lipid, protein which could be via acetyl coenzyme A to form a common metabolic pathway - tricarboxylic acid circulation and phosphorylation. Through this path, Acetyl-CoA thoroughly could be oxidize carbon dioxide and water, and release energy for ATP synthesis. Further, Acetyl-CoA a is a precursor material while synthetic fatty acid, ketone body, and Cholesterol and its derivatives.
Product Categories/Family for Acetyl-CoA assay kit
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Product Notes
The Acetyl-CoA (Catalog #AAA9719208) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Acetyl Coenzyme A (Acetyl-CoA), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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