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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse Dickkopf-related protein 2 (DKK2) Standard Curve.)

Mouse Dickkopf-Related Protein 2 (DKK2) ELISA Kit | DKK2 elisa kit

Mouse Dickkopf-Related Protein 2 (DKK2) ELISA Kit

Reactivity
Mouse
Synonyms
Dickkopf-Related Protein 2 (DKK2); Mouse Dickkopf-Related Protein 2 (DKK2) ELISA Kit; DKK2; DKK-2; dickkopf 2; dickkopf homolog 2; dickkopf related protein-2; DKK2 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
Mouse Dickkopf-related protein 2 (DKK2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DKK2. No significant cross-reactivity or interference between Mouse DKK2 and analogues was observed.
Sequence Length
259
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Dickkopf-related protein 2 (DKK2) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse Dickkopf-related protein 2 (DKK2) Standard Curve.)
Related Product Information for DKK2 elisa kit
Description: This Mouse Dickkopf-related protein 2 (DKK2) ELISA Kit employs a two-site sandwich ELISA to quantitate DKK2 in samples. An antibody specific for DKK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DKK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DKK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DKK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Dickkopf-related protein 2 is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6).Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dickkopf-related protein 2
NCBI Official Synonym Full Names
dickkopf WNT signaling pathway inhibitor 2
NCBI Official Symbol
Dkk2
NCBI Protein Information
dickkopf-related protein 2
UniProt Protein Name
Dickkopf-related protein 2
Protein Family
UniProt Gene Name
Dkk2
UniProt Synonym Gene Names
Dickkopf-2; Dkk-2; mDkk-2
UniProt Entry Name
DKK2_MOUSE

Uniprot Description

DKK2: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP5 and LRP6. Expressed in heart, brain, skeletal muscle and lung. Belongs to the dickkopf family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; multicellular organismal development

Research Articles on DKK2

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Product Notes

The Mouse DKK2 dkk2 (Catalog #AAA9717873) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717873 ELISA Kit recognizes Mouse DKK2. It is sometimes possible for the material contained within the vial of "Dickkopf-Related Protein 2 (DKK2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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