Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dickkopf-related protein 4 (DKK4) Standard Curve.)
Human Dickkopf-Related Protein 4 (DKK4) ELISA Kit | DKK4 elisa kit
Human Dickkopf-Related Protein 4 (DKK4) ELISA Kit
Gene Names
DKK4; DKK-4
Reactivity
Human
Synonyms
Dickkopf-Related Protein 4 (DKK4); Human Dickkopf-Related Protein 4 (DKK4) ELISA Kit; DKK4; DKK-4; MGC129562; MGC129563; dickkopf homolog 4; DKK4 elisa kit
Reactivity
Human
Specificity
Human Dickkopf-related protein 4 (DKK4) ELISA Kit has high sensitivity and excellent specificity for detection of Human DKK4. No significant cross-reactivity or interference between Human DKK4 and analogues was observed.
Sequence Length
224
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dickkopf-related protein 4 (DKK4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dickkopf-related protein 4 (DKK4) Standard Curve.)
Related Product Information for DKK4 elisa kit
Description: This Human Dickkopf-related protein 4 (DKK4) ELISA Kit employs a two-site sandwich ELISA to quantitate DKK4 in samples. An antibody specific for DKK4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DKK4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DKK4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DKK4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. Western blot analysis showed that DKK4 is secreted as 40-, 30- to 32-, and 15- to 17-kD proteins; these proteins were not significantly affected by N-glycanase treatment. N-terminal sequence analysis of the different forms suggested that DKK4 is proteolytically processed, possibly by intracellular furin-type proteases. Functional analysis determined that DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.
Background: All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. Western blot analysis showed that DKK4 is secreted as 40-, 30- to 32-, and 15- to 17-kD proteins; these proteins were not significantly affected by N-glycanase treatment. N-terminal sequence analysis of the different forms suggested that DKK4 is proteolytically processed, possibly by intracellular furin-type proteases. Functional analysis determined that DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dickkopf-related protein 4
NCBI Official Synonym Full Names
dickkopf WNT signaling pathway inhibitor 4
NCBI Official Symbol
DKK4
NCBI Official Synonym Symbols
DKK-4
NCBI Protein Information
dickkopf-related protein 4
UniProt Protein Name
Dickkopf-related protein 4
Protein Family
UniProt Gene Name
DKK4
UniProt Synonym Gene Names
Dickkopf-4; Dkk-4; hDkk-4
NCBI Description
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. [provided by RefSeq, Jul 2008]
Uniprot Description
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease ().
Research Articles on DKK4
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Product Notes
The Human DKK4 dkk4 (Catalog #AAA9715701) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715701 ELISA Kit recognizes Human DKK4. It is sometimes possible for the material contained within the vial of "Dickkopf-Related Protein 4 (DKK4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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