Rat BACE2 (Beta-secretase 2) ELISA Kit | BACE2 elisa kit

Rat BACE2 (Beta-secretase 2) ELISA Kit

Cat. Num. AAA7607291

• Gene Names: Bace2; ARP1; ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1; AI850424; 1110059C24Rik
• Reactivity: Rat
• Synonyms: BACE2 (Beta-secretase 2); Rat BACE2 (Beta-secretase 2) ELISA Kit; BACE2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Abeta42. No significant cross-reactivity or interference between Abeta42 and analogues was observed.
• Sequence Length: 514

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 3.906-250pg/ml
• Sensitivity: <2.344pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Abeta 42 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Abeta 42 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BACE2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Abeta42 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Abeta42 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Abeta42 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Abeta42 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 244798416
• NCBI GeneID: 56175
• NCBI Accession #: NP_062390.3
• NCBI GenBank Nucleotide #: NM_019517.5
• UniProt Accession #: Q9JL18; Q3TNS7; Q8C5E9; Q8C793; Q9R1P7
• Molecular Weight: 55,800 Da
• NCBI Official Full Name: beta-secretase 2 preproprotein
• NCBI Official Synonym Full Names: beta-site APP-cleaving enzyme 2
• NCBI Official Symbol: Bace2
• NCBI Official Synonym Symbols: ARP1; ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1; AI850424; 1110059C24Rik
• NCBI Protein Information: beta-secretase 2
• UniProt Protein Name: Beta-secretase 2
• Protein Family: Beta-secretase
• UniProt Gene Name: Bace2
• UniProt Synonym Gene Names: ASP1; Asp 1; Beta-site APP cleaving enzyme 2

NCBI Description

This gene encodes a member of the peptidase A1 family of aspartic proteases. The encoded preproprotein undergoes proteolytic processing to generate an active endopeptidase enzyme. This transmembrane protease catalyzes the proteolysis of amyloid precursor protein to produce amyloid beta peptide. Mice lacking the encoded product exhibit increased pancreatic beta cell mass and improved glucose tolerance due to increased insulin secretion. [provided by RefSeq, Jul 2016]

Uniprot Description

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672 (). Responsible also for the proteolytic processing of CLTRN in pancreatic beta cells ().