Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dickkopf-related protein 1 (DKK1) Standard Curve.)
Human Dickkopf-Related Protein 1 (DKK1) ELISA Kit | DKK1 elisa kit
Human Dickkopf-Related Protein 1 (DKK1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dickkopf-related protein 1 (DKK1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dickkopf-related protein 1 (DKK1) Standard Curve.)
Background: Dickkopf-related protein 1 is a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Sequence analysis predicted that the 266-amino acid DKK1 protein contains a 31-amino acid N-terminal signal peptide, 2 clusters of cysteine residues, and a potential C-terminal N-glycosylation site. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kD doublet protein, which is larger than the deduced molecular mass of 26 kD.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines. [provided by RefSeq, Sep 2017]
Uniprot Description
DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.
Protein type: Secreted; Secreted, signal peptide; Inhibitor
Chromosomal Location of Human Ortholog: 10q11.2
Cellular Component: extracellular space; early endosome membrane; extracellular region; plasma membrane
Molecular Function: protein binding; receptor antagonist activity; signal transducer activity; growth factor activity; low-density lipoprotein receptor binding
Biological Process: cellular morphogenesis during differentiation; negative regulation of skeletal muscle development; response to retinoic acid; negative regulation of Wnt receptor signaling pathway; regulation of receptor internalization; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; negative regulation of mesodermal cell fate specification; mesoderm formation; hair follicle development; endoderm formation; forebrain development; regulation of endodermal cell fate specification; negative regulation of protein complex assembly; embryonic limb morphogenesis
Research Articles on DKK1
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Product Notes
The Human DKK1 dkk1 (Catalog #AAA9715704) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715704 ELISA Kit recognizes Human DKK1. It is sometimes possible for the material contained within the vial of "Dickkopf-Related Protein 1 (DKK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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