Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Dystrophia myotonica WD repeat-containing protein (DMWD) Standard Curve.)
Mouse Dystrophia Myotonica WD Repeat-Containing Protein (DMWD) ELISA Kit | DMWD elisa kit
Mouse Dystrophia Myotonica WD Repeat-Containing Protein (DMWD) ELISA Kit
Gene Names
Dmwd; Dm9; DMR-N9
Reactivity
Mouse
Synonyms
Dystrophia Myotonica WD Repeat-Containing Protein (DMWD); Mouse Dystrophia Myotonica WD Repeat-Containing Protein (DMWD) ELISA Kit; DMWD; D19S593E; DMR-N9; DMRN9; gene59; dystrophia myotonica-containing WD repeat motif; dystrophia myotonica-containing WD repeat motif protein; protein 59; DMWD elisa kit
Reactivity
Mouse
Specificity
Mouse Dystrophia myotonica WD repeat-containing protein (DMWD) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DMWD. No significant cross-reactivity or interference between Mouse DMWD and analogues was observed.
Sequence Length
665
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Dystrophia myotonica WD repeat-containing protein (DMWD) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Dystrophia myotonica WD repeat-containing protein (DMWD) Standard Curve.)
Related Product Information for DMWD elisa kit
Description: This Mouse Dystrophia myotonica WD repeat-containing protein (DMWD) ELISA Kit employs a two-site sandwich ELISA to quantitate DMWD in samples. An antibody specific for DMWD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DMWD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMWD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMWD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DMWD contains 2 regions with significant homology to WD repeats. Northern blot analysis detected highest expression in mouse brain and testis, with significant expression in heart and lung. In situ hybridization of mouse embryos detected ubiquitous low expression in all tissues and enhanced expression in adult brain and testis. Dmwd expression began before embryonic day 9.5. From day 14.5 onward, Dmwd mRNA was detected in all neural tissues, especially in the telencephalon and mesencephalon. Later, expression was evident in distinct tubules of the mature testis and was restricted to secondary spermatocytes of stages VIII to XII of the spermatogenic proliferation cycle.Northern blot analysis of human and baboon RNA detected a 3-kb transcript.
Background: DMWD contains 2 regions with significant homology to WD repeats. Northern blot analysis detected highest expression in mouse brain and testis, with significant expression in heart and lung. In situ hybridization of mouse embryos detected ubiquitous low expression in all tissues and enhanced expression in adult brain and testis. Dmwd expression began before embryonic day 9.5. From day 14.5 onward, Dmwd mRNA was detected in all neural tissues, especially in the telencephalon and mesencephalon. Later, expression was evident in distinct tubules of the mature testis and was restricted to secondary spermatocytes of stages VIII to XII of the spermatogenic proliferation cycle.Northern blot analysis of human and baboon RNA detected a 3-kb transcript.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dystrophia myotonica WD repeat-containing protein
NCBI Official Synonym Full Names
dystrophia myotonica-containing WD repeat motif
NCBI Official Symbol
Dmwd
NCBI Official Synonym Symbols
Dm9; DMR-N9
NCBI Protein Information
dystrophia myotonica WD repeat-containing protein
UniProt Protein Name
Dystrophia myotonica WD repeat-containing protein
Protein Family
UniProt Gene Name
Dmwd
UniProt Synonym Gene Names
Dm9
UniProt Entry Name
DMWD_MOUSE
Uniprot Description
DMWD: Could have a regulatory function in meiosis.
Research Articles on DMWD
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Product Notes
The Mouse DMWD dmwd (Catalog #AAA9717841) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717841 ELISA Kit recognizes Mouse DMWD. It is sometimes possible for the material contained within the vial of "Dystrophia Myotonica WD Repeat-Containing Protein (DMWD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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