Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DmX-like protein 2 (DMXL2) Standard Curve.)
Human DmX-Like Protein 2 (DMXL2) ELISA Kit | DMXL2 elisa kit
Human DmX-Like Protein 2 (DMXL2) ELISA Kit
Gene Names
DMXL2; RC3; PEPNS; DFNA71
Reactivity
Human
Synonyms
DmX-Like Protein 2 (DMXL2); Human DmX-Like Protein 2 (DMXL2) ELISA Kit; DMXL2; FLJ26672; KIAA0856; RC3; rabconnectin 3; rabconnectin-3; DMXL2 elisa kit
Reactivity
Human
Specificity
Human DmX-like protein 2 (DMXL2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DMXL2. No significant cross-reactivity or interference between Human DMXL2 and analogues was observed.
Sequence Length
3037
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DmX-like protein 2 (DMXL2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DmX-like protein 2 (DMXL2) Standard Curve.)
Related Product Information for DMXL2 elisa kit
Description: This Human DmX-like protein 2 (DMXL2) ELISA Kit employs a two-site sandwich ELISA to quantitate DMXL2 in samples. An antibody specific for DMXL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DMXL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMXL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMXL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DMXL2 shares significant homology with DMXL1. RT-PCR ELISA detected moderate expression in brain, lung, liver, kidney, testis, and ovary. Expression was low in heart and undetectable in skeletal muscle, pancreas, and spleen. The deduced 3,036-amino acid protein has a calculated molecular mass of 339.8 kD and contains 12 WD domains. Western blot analysis of rat tissues showed abundant expression of Dmxl2 in brain only, and subcellular distribution analysis revealed enrichment in the synaptic vesicle fraction. Immunofluorescence and immunoelectron microscopy of mouse hippocampus and primary cultured rat hippocampal neurons showed that Dmxl2 was concentrated on synaptic vesicles at synapses.
Background: DMXL2 shares significant homology with DMXL1. RT-PCR ELISA detected moderate expression in brain, lung, liver, kidney, testis, and ovary. Expression was low in heart and undetectable in skeletal muscle, pancreas, and spleen. The deduced 3,036-amino acid protein has a calculated molecular mass of 339.8 kD and contains 12 WD domains. Western blot analysis of rat tissues showed abundant expression of Dmxl2 in brain only, and subcellular distribution analysis revealed enrichment in the synaptic vesicle fraction. Immunofluorescence and immunoelectron microscopy of mouse hippocampus and primary cultured rat hippocampal neurons showed that Dmxl2 was concentrated on synaptic vesicles at synapses.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dmX-like protein 2 isoform 1
NCBI Official Synonym Full Names
Dmx like 2
NCBI Official Symbol
DMXL2
NCBI Official Synonym Symbols
RC3; PEPNS; DFNA71
NCBI Protein Information
dmX-like protein 2
UniProt Protein Name
DmX-like protein 2
Protein Family
UniProt Gene Name
DMXL2
UniProt Synonym Gene Names
KIAA0856
UniProt Entry Name
DMXL2_HUMAN
NCBI Description
This gene encodes a protein with 12 WD domains. Proteins with WD domains are involved in many functions including participation in signal transduction pathways. Participation of the encoded protein in regulation of the Notch signaling pathway has been demonstrated in vitro using several human cell lines (PMID:20810660). A gene encoding a similar protein is located on chromosome 5. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
RC3: May serve as a scaffold protein for MADD and RAB3GA on synaptic vesicles. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Vesicle; Membrane protein, peripheral
Chromosomal Location of Human Ortholog: 15q21.2
Cellular Component: extracellular space; synaptic vesicle; synaptic vesicle membrane; cell junction
Molecular Function: Rab GTPase binding
Disease: Polyendocrine-polyneuropathy Syndrome
Research Articles on DMXL2
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Product Notes
The Human DMXL2 dmxl2 (Catalog #AAA9715667) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715667 ELISA Kit recognizes Human DMXL2. It is sometimes possible for the material contained within the vial of "DmX-Like Protein 2 (DMXL2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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