Mouse Myotonin-Protein Kinase (DMPK) ELISA Kit | DMPK elisa kit

Mouse Myotonin-Protein Kinase (DMPK) ELISA Kit

Cat. Num. AAA9717850

• Gene Names: Dmpk; DM; DMK; Dm15; MDPK; MT-PK
• Reactivity: Mouse
• Synonyms: Myotonin-Protein Kinase (DMPK); Mouse Myotonin-Protein Kinase (DMPK) ELISA Kit; DMPK; DM; DM1; DM1PK; DMK; MDPK; MT-PK; DM protein kinase; myotonic dystrophy associated protein kinase; myotonic dystrophy protein kinase; myotonin protein kinase A; thymopoietin homolog; DMPK elisa kit

• Reactivity: Mouse
• Specificity: Mouse Myotonin-protein kinase (DMPK) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DMPK. No significant cross-reactivity or interference between Mouse DMPK and analogues was observed.
• Sequence Length: 537

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Myotonin-protein kinase (DMPK) Standard Curve.)

Related Product Information for DMPK elisa kit

Description: This Mouse Myotonin-protein kinase (DMPK) ELISA Kit employs a two-site sandwich ELISA to quantitate DMPK in samples. An antibody specific for DMPK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DMPK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMPK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMPK bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Myotonic dystrophy protein kinase is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

NCBI and Uniprot Product Information

• NCBI GI #: 299758507
• NCBI GeneID: 13400
• NCBI Accession #: NP_001177420.1
• NCBI GenBank Nucleotide #: NP_001177420.1
• UniProt Accession #: P54265
• NCBI Official Full Name: myotonin-protein kinase isoform 3
• NCBI Official Synonym Full Names: dystrophia myotonica-protein kinase
• NCBI Official Symbol: Dmpk
• NCBI Official Synonym Symbols: DM; DMK; Dm15; MDPK; MT-PK
• NCBI Protein Information: myotonin-protein kinase
• UniProt Protein Name: Myotonin-protein kinase
• Protein Family: Myotonin-protein kinase
• UniProt Gene Name: Dmpk
• UniProt Synonym Gene Names: Dm15; Mdpk; MT-PK; DMK; MDPK
• UniProt Entry Name: DMPK_MOUSE

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase that contains coiled-coil and C-terminal membrane association domains. In the embryonic mouse, it is found in cardiac and skeletal myocytes where it appears to play a role in myogenesis. In adults, the transcript is localized to several tissues including brain, heart, and skeletal and smooth muscle, and a function in cytoskeletal remodeling has been described. Transcripts with expanded CUG repeats in the 3' untranslated region mediate alternative splicing of several genes and sequester RNA binding proteins and RNA transcripts that contain CAG repeats, resulting in myotonic dystrophy, an autosomal dominant neuromuscular disorder. Alternative splicing results in multiple protein coding and non-coding transcript variants. [provided by RefSeq, Oct 2014]