Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) Standard Curve.)
Mouse CAMP-Dependent Protein Kinase Type II-beta Regulatory Subunit (PRKAR2B) ELISA Kit | PRKAR2B elisa kit
Mouse CAMP-Dependent Protein Kinase Type II-beta Regulatory Subunit (PRKAR2B) ELISA Kit
Gene Names
Prkar2b; Pkarb2; AI451071; AW061005; RII(beta); PKARIIbeta
Reactivity
Mouse
Synonyms
CAMP-Dependent Protein Kinase Type II-beta Regulatory Subunit (PRKAR2B); Mouse CAMP-Dependent Protein Kinase Type II-beta Regulatory Subunit (PRKAR2B) ELISA Kit; PRKAR2B; PRKAR2; RII-BETA; H_RG363E19.2; WUGSC:H_RG363E19.2; cAMP-dependent protein kinase type-beta regulatory chain; cAMP-dependent protein kinase; regulatory subunit beta 2; PRKAR2B elisa kit
Reactivity
Mouse
Specificity
Mouse CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRKAR2B. No significant cross-reactivity or interference between Mouse PRKAR2B and analogues was observed.
Sequence Length
416
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) Standard Curve.)
Related Product Information for PRKAR2B elisa kit
Description: This Mouse CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR2B in samples. An antibody specific for PRKAR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRKAR2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAR2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PRKAR2b is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity.
Background: The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PRKAR2b is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
cAMP-dependent protein kinase type II-beta regulatory subunit
NCBI Official Synonym Full Names
protein kinase, cAMP dependent regulatory, type II beta
NCBI Official Symbol
Prkar2b
NCBI Official Synonym Symbols
Pkarb2; AI451071; AW061005; RII(beta); PKARIIbeta
NCBI Protein Information
cAMP-dependent protein kinase type II-beta regulatory subunit
UniProt Protein Name
cAMP-dependent protein kinase type II-beta regulatory subunit
Protein Family
UniProt Gene Name
Prkar2b
UniProt Entry Name
KAP3_MOUSE
Uniprot Description
PKAR2B: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Protein type: Protein kinase, regulatory subunit; Mitochondrial
Cellular Component: cAMP-dependent protein kinase complex; cell soma; centrosome; cytoplasm; dendrite; dendritic shaft; dendritic spine; lipid raft; membrane; mitochondrial inner membrane; perinuclear region of cytoplasm; plasma membrane
Molecular Function: cAMP binding; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; nucleotide binding; protein binding; protein domain specific binding; protein kinase binding; ubiquitin protein ligase binding
Biological Process: fatty acid metabolic process; learning; regulation of protein amino acid phosphorylation; regulation of protein kinase activity
Research Articles on PRKAR2B
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Product Notes
The Mouse PRKAR2B prkar2b (Catalog #AAA9717277) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717277 ELISA Kit recognizes Mouse PRKAR2B. It is sometimes possible for the material contained within the vial of "CAMP-Dependent Protein Kinase Type II-beta Regulatory Subunit (PRKAR2B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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