Mouse CAMP-Dependent Protein Kinase Catalytic Subunit alpha (PRKACA) ELISA Kit | PRKACA elisa kit

Mouse CAMP-Dependent Protein Kinase Catalytic Subunit alpha (PRKACA) ELISA Kit

Cat. Num. AAA9717282

• Gene Names: Prkaca; PKCD; Pkaca
• Reactivity: Mouse
• Synonyms: CAMP-Dependent Protein Kinase Catalytic Subunit alpha (PRKACA); Mouse CAMP-Dependent Protein Kinase Catalytic Subunit alpha (PRKACA) ELISA Kit; PRKACA; MGC102831; MGC48865; PKACA; PKA C-alpha; cAMP-dependent protein kinase catalytic subunit alpha; isoform 1; protein kinase A catalytic subunit; PRKACA elisa kit

• Reactivity: Mouse
• Specificity: Mouse CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRKACA. No significant cross-reactivity or interference between Mouse PRKACA and analogues was observed.
• Sequence Length: 343

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) Standard Curve.)

Related Product Information for PRKACA elisa kit

Description: This Mouse CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACA in samples. An antibody specific for PRKACA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRKACA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKACA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKACA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the AMP-activated protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PRKACais a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

NCBI and Uniprot Product Information

• NCBI GI #: 483968030
• NCBI GeneID: 18747
• NCBI Accession #: NP_001264827.1
• NCBI GenBank Nucleotide #: NP_001264827.1
• UniProt Accession #: P05132
• NCBI Official Full Name: cAMP-dependent protein kinase catalytic subunit alpha isoform 2
• NCBI Official Synonym Full Names: protein kinase, cAMP dependent, catalytic, alpha
• NCBI Official Symbol: Prkaca
• NCBI Official Synonym Symbols: PKCD; Pkaca
• NCBI Protein Information: cAMP-dependent protein kinase catalytic subunit alpha
• UniProt Protein Name: cAMP-dependent protein kinase catalytic subunit alpha
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: Prkaca
• UniProt Synonym Gene Names: Pkaca; PKA C-alpha
• UniProt Entry Name: KAPCA_MOUSE

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The holoenzyme, protein kinase A (also known as cyclic-AMP dependent protein kinase), mediates cellular response to changes in cyclic-AMP levels. This gene encodes the alpha catalytic subunit of protein kinase A. Protein kinase A-mediated signaling is transduced via phosphorylation of target proteins, and is important for many cellular functions, including mammalian sperm maturation and motility. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. [provided by RefSeq, Apr 2013]

Uniprot Description

PKACA: catalytic subunit of cAMP-dependent protein kinase alpha, an AGC kinase. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Two splice-variant isoforms have been described.

Protein type: Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.11; AGC group; PKA family

Cellular Component: Golgi apparatus; centrosome; neuron projection; protein complex; mitochondrion; cilium; cAMP-dependent protein kinase complex; cell projection; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus; neuromuscular junction; AMP-activated protein kinase complex

Molecular Function: cAMP-dependent protein kinase activity; nucleotide binding; protein kinase binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; transferase activity, transferring phosphorus-containing groups; protein serine/threonine/tyrosine kinase activity; protein complex binding; kinase activity; ATP binding; Rab GTPase binding

Biological Process: regulation of synaptic transmission; regulation of synaptic transmission, glutamatergic; regulation of osteoblast differentiation; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; negative regulation of meiotic cell cycle; protein amino acid phosphorylation; regulation of cellular respiration; positive regulation of protein export from nucleus; peptidyl-serine phosphorylation; mesoderm formation; neural tube closure; positive regulation of cell proliferation; phosphorylation; sperm capacitation