Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat CAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) Standard Curve.)
Rat CAMP-Dependent Protein Kinase Type I-alpha Regulatory Subunit (PRKAR1A) ELISA Kit | PRKAR1A elisa kit
Rat CAMP-Dependent Protein Kinase Type I-alpha Regulatory Subunit (PRKAR1A) ELISA Kit
Gene Names
Prkar1a; RIIA
Reactivity
Rat
Synonyms
CAMP-Dependent Protein Kinase Type I-alpha Regulatory Subunit (PRKAR1A); Rat CAMP-Dependent Protein Kinase Type I-alpha Regulatory Subunit (PRKAR1A) ELISA Kit; PRKAR1A; CAR; CNC; CNC1; DKFZp779L0468; MGC17251; PKR1; PPNAD1; PRKAR1; TSE1; cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain; cAMP-depende; PRKAR1A elisa kit
Reactivity
Rat
Specificity
Rat CAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRKAR1A. No significant cross-reactivity or interference between Rat PRKAR1A and analogues was observed.
Sequence Length
381
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat CAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat CAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) Standard Curve.)
Related Product Information for PRKAR1A elisa kit
Description: This Rat CAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR1A in samples. An antibody specific for PRKAR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRKAR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAR1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase A (PKA), which transduces the signal through phosphorylation of different target proteins. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. TSE1 is one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Functional null mutations in this gene cause Carney complex (CNC), an autosomal dominant multiple neoplasia syndrome. This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2.
Background: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase A (PKA), which transduces the signal through phosphorylation of different target proteins. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. TSE1 is one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Functional null mutations in this gene cause Carney complex (CNC), an autosomal dominant multiple neoplasia syndrome. This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cAMP-dependent protein kinase type I-alpha regulatory subunit
NCBI Official Synonym Full Names
protein kinase cAMP-dependent type I regulatory subunit alpha
NCBI Official Symbol
Prkar1a
NCBI Official Synonym Symbols
RIIA
NCBI Protein Information
cAMP-dependent protein kinase type I-alpha regulatory subunit
UniProt Protein Name
cAMP-dependent protein kinase type I-alpha regulatory subunit
Protein Family
UniProt Gene Name
Prkar1a
UniProt Entry Name
KAP0_RAT
NCBI Description
regulatory subunit of cAMP-dependent protein kinase (PKA); negatively regulates meiosis [RGD, Feb 2006]
Research Articles on PRKAR1A
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Product Notes
The Rat PRKAR1A prkar1a (Catalog #AAA9712073) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712073 ELISA Kit recognizes Rat PRKAR1A. It is sometimes possible for the material contained within the vial of "CAMP-Dependent Protein Kinase Type I-alpha Regulatory Subunit (PRKAR1A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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