Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) Standard Curve.)
Mouse CAMP-Dependent Protein Kinase Type II-alpha Regulatory Subunit (PRKAR2A) ELISA Kit | PRKAR2A elisa kit
Mouse CAMP-Dependent Protein Kinase Type II-alpha Regulatory Subunit (PRKAR2A) ELISA Kit
Gene Names
Prkar2a; AI317181; AI836829; RII(alpha); 1110061A24Rik
Reactivity
Mouse
Synonyms
CAMP-Dependent Protein Kinase Type II-alpha Regulatory Subunit (PRKAR2A); Mouse CAMP-Dependent Protein Kinase Type II-alpha Regulatory Subunit (PRKAR2A) ELISA Kit; PRKAR2A; MGC3606; PKR2; PRKAR2; OTTHUMP00000210266; cAMP-dependent protein kinase regulatory subunit RII alpha; cAMP-dependent protein kinase; regulatory subunit alpha 2; protein kinase A; RII-alpha subunit; PRKAR2A elisa kit
Reactivity
Mouse
Specificity
Mouse CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRKAR2A. No significant cross-reactivity or interference between Mouse PRKAR2A and analogues was observed.
Sequence Length
401
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) Standard Curve.)
Related Product Information for PRKAR2A elisa kit
Description: This Mouse CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR2A in samples. An antibody specific for PRKAR2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRKAR2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAR2A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR2A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The inactive holoenzyme of PKA is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. PRKAR2a is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of PKA. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
Background: The inactive holoenzyme of PKA is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. PRKAR2a is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of PKA. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
cAMP-dependent protein kinase type II-alpha regulatory subunit
NCBI Official Synonym Full Names
protein kinase, cAMP dependent regulatory, type II alpha
NCBI Official Symbol
Prkar2a
NCBI Official Synonym Symbols
AI317181; AI836829; RII(alpha); 1110061A24Rik
NCBI Protein Information
cAMP-dependent protein kinase type II-alpha regulatory subunit
UniProt Protein Name
cAMP-dependent protein kinase type II-alpha regulatory subunit
Protein Family
UniProt Gene Name
Prkar2a
UniProt Entry Name
KAP2_MOUSE
Research Articles on PRKAR2A
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Product Notes
The Mouse PRKAR2A prkar2a (Catalog #AAA9717278) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717278 ELISA Kit recognizes Mouse PRKAR2A. It is sometimes possible for the material contained within the vial of "CAMP-Dependent Protein Kinase Type II-alpha Regulatory Subunit (PRKAR2A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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