Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Acetylcholinesterase (ACHE) Standard Curve.)
Mouse Acetylcholinesterase (ACHE) ELISA Kit | ACHE elisa kit
Mouse Acetylcholinesterase (ACHE) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Acetylcholinesterase (ACHE) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Acetylcholinesterase (ACHE) Standard Curve.)
Background: Acetylcholinesterase is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission. AChE has a very high catalytic activity mdash; each molecule of AChE degrades about 5000 molecules of acetylcholine per second. The choline produced by the action of AChE is recycled mdash; it is transported, through reuptake, back into nerve terminals where it is used to synthesize new acetylcholine molecules.Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen.
NCBI and Uniprot Product Information
Uniprot Description
AChE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.1.7; Hydrolase; Lipid Metabolism - glycerophospholipid; Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 5 G2|5 76.32 cM
Cellular Component: axon; basal lamina; cell junction; cell soma; cell surface; cytoplasm; dendrite; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi apparatus; membrane; neuromuscular junction; neuron projection; nuclear envelope; perinuclear region of cytoplasm; plasma membrane; postsynaptic membrane; presynaptic membrane; rough endoplasmic reticulum; synapse
Molecular Function: acetylcholine binding; acetylcholinesterase activity; choline binding; cholinesterase activity; collagen binding; hydrolase activity; laminin binding; protein binding; protein homodimerization activity; protein self-association; serine hydrolase activity
Biological Process: acetylcholine catabolic process; acetylcholine metabolic process; cell adhesion; choline metabolic process; neurotransmitter catabolic process; neurotransmitter receptor biosynthetic process; positive regulation of axonogenesis; positive regulation of dendrite morphogenesis; protein tetramerization; receptor internalization; regulation of receptor recycling; retina development in camera-type eye; synaptogenesis
Research Articles on ACHE
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Product Notes
The Mouse ACHE ache (Catalog #AAA9717148) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717148 ELISA Kit recognizes Mouse ACHE. It is sometimes possible for the material contained within the vial of "Acetylcholinesterase (ACHE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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