Western Blot (WB)
(Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human Jurkat Cells; Lane3: Human K562 Cells; Lane4: Human Hela Cells.)
Rabbit anti-Human Malic Enzyme 2, NADP+ Dependent Polyclonal Antibody | anti-ME2 antibody
Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human Jurkat Cells; Lane3: Human K562 Cells; Lane4: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human Jurkat Cells; Lane3: Human K562 Cells; Lane4: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ME2, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ME2, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Glioma Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Glioma Tissue)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]
Uniprot Description
ME2: a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]
Protein type: Carbohydrate Metabolism - pyruvate; EC 1.1.1.38; Mitochondrial; Oxidoreductase
Chromosomal Location of Human Ortholog: 18q21.2
Cellular Component: mitochondrial matrix; mitochondrion
Molecular Function: electron transfer activity; malate dehydrogenase (decarboxylating) activity; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; malic enzyme activity; metal ion binding; NAD binding; oxaloacetate decarboxylase activity
Biological Process: malate metabolic process; pyruvate metabolic process; regulation of NADP metabolic process
Disease: Epilepsy, Idiopathic Generalized; Opioid Dependence, Susceptibility To, 1
Research Articles on ME2
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Product Notes
The ME2 me2 (Catalog #AAA2032642) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Malic Enzyme 2, NADP+ Dependent can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ME2 me2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Malic Enzyme 2, NADP+ Dependent, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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