Western Blot (WB)
(Western blot analysis of ITGBL1 Antibody (Center) in Hela cell line lysates (35ug/lane). ITGBL1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human Integrin beta-like 1 with EGF-like Repeat Domains Polyclonal Antibody | anti-ITGBL1 antibody
Integrin beta-like 1 with EGF-like Repeat Domains (Integrin beta-like Protein 1, ITGBL1, Osteoblast-specific Cysteine-rich Protein, OSCP, Ten Integrin EGF-like Repeat Domain-containing Protein, TIED) (AP)
Gene Names
ITGBL1; OSCP; TIED
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Immunoaffinity chromatography.
Synonyms
Integrin beta-like 1 with EGF-like Repeat Domains; Polyclonal Antibody; Integrin beta-like 1 with EGF-like Repeat Domains (Integrin beta-like Protein 1; ITGBL1; Osteoblast-specific Cysteine-rich Protein; OSCP; Ten Integrin EGF-like Repeat Domain-containing Protein; TIED) (AP); anti-ITGBL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ITGBL1.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-ITGBL1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the center region of human ITGBL1 (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ITGBL1 Antibody (Center) in Hela cell line lysates (35ug/lane). ITGBL1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ITGBL1 Antibody (Center) in Hela cell line lysates (35ug/lane). ITGBL1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with ITGBL1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with ITGBL1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(ITGBL1 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ITGBL1 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-ITGBL1 antibody
References
1. Berg R.W., et.al., Genomics 56:169-178(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,921 Da
NCBI Official Full Name
integrin beta-like protein 1 isoform 1
NCBI Official Synonym Full Names
integrin, beta-like 1 (with EGF-like repeat domains)
NCBI Official Symbol
ITGBL1
NCBI Official Synonym Symbols
OSCP; TIED
NCBI Protein Information
integrin beta-like protein 1; osteoblast-specific cysteine-rich protein; ten integrin EGF-like repeat domain-containing protein
UniProt Protein Name
Integrin beta-like protein 1
Protein Family
UniProt Gene Name
ITGBL1
UniProt Synonym Gene Names
OSCP; TIED
UniProt Entry Name
ITGBL_HUMAN
NCBI Description
This gene encodes a beta integrin-related protein that is a member of the EGF-like protein family. The encoded protein contains integrin-like cysteine-rich repeats. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
Uniprot Description
ITGBL1: 3 isoforms of the human protein are produced by alternative splicing
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Cell adhesion
Chromosomal Location of Human Ortholog: 13q33
Cellular Component: extracellular region
Biological Process: cell adhesion
Research Articles on ITGBL1
Similar Products
Product Notes
The ITGBL1 itgbl1 (Catalog #AAA6482072) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Integrin beta-like 1 with EGF-like Repeat Domains (Integrin beta-like Protein 1, ITGBL1, Osteoblast-specific Cysteine-rich Protein, OSCP, Ten Integrin EGF-like Repeat Domain-containing Protein, TIED) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin beta-like 1 with EGF-like Repeat Domains can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-1:500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ITGBL1 itgbl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin beta-like 1 with EGF-like Repeat Domains, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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