Mouse Acetylcholinesterase (AChE) ELISA Kit | AChE elisa kit

Mouse Acetylcholinesterase, AChE ELISA Kit

Cat. Num. AAA944406

• Reactivity: Mouse
• Synonyms: Acetylcholinesterase (AChE); Mouse Acetylcholinesterase; AChE ELISA Kit; Mouse Acetylcholinesterase (AChE) ELISA Kit; ARACHE; N-ACHE; YT; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase; AChE elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse AChE. No significant cross-reactivity or interference between mouse AChE and analogues was observed.
• Sequence Length: 614

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 0.312 mU/ml -20 mU/mL
• Sensitivity: <0.078 mU/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AChE elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AChE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AChE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AChE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AChE bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 586597902
• NCBI GeneID: 11423
• NCBI Accession #: NP_001276939.1
• NCBI GenBank Nucleotide #: NM_001290010.1
• UniProt Accession #: P21836
• Molecular Weight: 68,169 Da
• NCBI Official Full Name: acetylcholinesterase
• NCBI Official Synonym Full Names: acetylcholinesterase
• NCBI Official Symbol: Ache
• NCBI Protein Information: acetylcholinesterase
• UniProt Protein Name: Acetylcholinesterase
• Protein Family: Acetylcholinesterase
• UniProt Gene Name: Ache
• UniProt Synonym Gene Names: AChE
• UniProt Entry Name: ACES_MOUSE

Uniprot Description

ACHE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.7; Lipid Metabolism - glycerophospholipid; Hydrolase; Membrane protein, GPI anchor

Cellular Component: Golgi apparatus; extracellular space; neuron projection; cell surface; rough endoplasmic reticulum; endoplasmic reticulum lumen; dendrite; extracellular region; nuclear envelope; presynaptic membrane; postsynaptic membrane; membrane; cell soma; perinuclear region of cytoplasm; axon; cytoplasm; plasma membrane; basal lamina; synapse; cell junction; neuromuscular junction

Molecular Function: collagen binding; choline binding; protein binding; serine hydrolase activity; protein homodimerization activity; cholinesterase activity; protein self-association; hydrolase activity; laminin binding; acetylcholinesterase activity; acetylcholine binding

Biological Process: neurotransmitter catabolic process; acetylcholine metabolic process; acetylcholine catabolic process; positive regulation of dendrite morphogenesis; synaptogenesis; choline metabolic process; retina development in camera-type eye; receptor internalization; neurotransmitter receptor biosynthetic process; regulation of receptor recycling; positive regulation of axonogenesis; cell adhesion; protein tetramerization