Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant HES1 protein (red) or empty vector (blue) stained using HES1 antibody)
Mouse HES1 Monoclonal Antibody | anti-HES1 antibody
HES1 antibody
Gene Names
C21orf33; ES1; HES1; KNPH; KNPI; GT335
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
HES1 antibody was purified by affinity chromatography.
Synonyms
HES1; Monoclonal Antibody; HES1 antibody; Monoclonal HES1; Anti-HES1; ; HES 1; HES-1; anti-HES1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1H8
Purity/Purification
HES1 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Sequence Length
268
Applicable Applications for anti-HES1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
WB: 1:2000,
IF: 1:100
WB: 1:2000,
Biological Significance
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
Cross-Reactivity
Human
Immunogen
HES1 antibody was raised in Mouse using full length human recombinant protein of human HES1 produced in HEK293T cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant HES1 protein (red) or empty vector (blue) stained using HES1 antibody)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant HES1 protein (red) or empty vector (blue) stained using HES1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HES1 protein (Right) or empty vector (Left) detected with HES1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HES1 protein (Right) or empty vector (Left) detected with HES1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant HES1 protein using HES1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant HES1 protein using HES1 antibody)
Related Product Information for anti-HES1 antibody
Mouse monoclonal HES1 antibody
Product Categories/Family for anti-HES1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
24,758 Da
NCBI Official Full Name
HES1
NCBI Official Synonym Full Names
chromosome 21 open reading frame 33
NCBI Official Symbol
C21orf33
NCBI Official Synonym Symbols
ES1; HES1; KNPH; KNPI; GT335
NCBI Protein Information
ES1 protein homolog, mitochondrial
UniProt Protein Name
ES1 protein homolog, mitochondrial
Protein Family
UniProt Gene Name
C21orf33
UniProt Synonym Gene Names
HES1; KNPI
UniProt Entry Name
ES1_HUMAN
NCBI Description
This gene encodes a potential mitochondrial protein that is a member of the DJ-1/PfpI gene family. This protein is overexpressed in fetal Down syndrome brain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
Uniprot Description
C21orf33: a mitochondrial protein. Two alternatively spliced isoforms have been described.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: mitochondrion
Research Articles on HES1
Similar Products
Product Notes
The HES1 c21orf33 (Catalog #AAA837993) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HES1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC: 1:100 IF: 1:100 WB: 1:2000,. Researchers should empirically determine the suitability of the HES1 c21orf33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HES1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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