Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sphingosine 1-phosphate receptor 2 (S1PR2) Standard Curve.)
Mouse Sphingosine 1-Phosphate Receptor 2 (S1PR2) ELISA Kit | S1PR2 elisa kit
Mouse Sphingosine 1-Phosphate Receptor 2 (S1PR2) ELISA Kit
Gene Names
S1pr2; Edg5; H218; LPb2; S1P2; Gpcr13; 1100001A16Rik
Reactivity
Mouse
Synonyms
Sphingosine 1-Phosphate Receptor 2 (S1PR2); Mouse Sphingosine 1-Phosphate Receptor 2 (S1PR2) ELISA Kit; S1PR2; AGR16; EDG-5; EDG5; Gpcr13; H218; LPB2; S1P2; S1P receptor EDG5; endothelial differentiation; sphingolipid G-protein-coupled receptor; 5; sphingosine 1-phosphate receptor 2; S1PR2 elisa kit
Reactivity
Mouse
Specificity
Mouse Sphingosine 1-phosphate receptor 2 (S1PR2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse S1PR2. No significant cross-reactivity or interference between Mouse S1PR2 and analogues was observed.
Sequence Length
352
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sphingosine 1-phosphate receptor 2 (S1PR2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sphingosine 1-phosphate receptor 2 (S1PR2) Standard Curve.)
Related Product Information for S1PR2 elisa kit
Description: This Mouse Sphingosine 1-phosphate receptor 2 (S1PR2) ELISA Kit employs a two-site sandwich ELISA to quantitate S1PR2 in samples. An antibody specific for S1PR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S1PR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S1PR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S1PR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The lysosphingolipid sphingosine 1-phosphate (S1P) regulates cell proliferation, apoptosis, motility, and neurite retraction. Its actions may be both intracellular as a second messenger and extracellular as a receptor ligand. S1P and the structurally related lysolipid mediator lysophosphatidic acid (LPA) signal cells through a set of G protein-coupled receptors known as EDG receptors. Some EDG receptors are S1P receptors; others are LPA receptors. According to these guidelines, a receptor is to be named with the abbreviation of the natural agonist with the highest potency, followed by a subscripted arabic number. Thus they suggested that the designation EDG5 should be changed to S1P2.
Background: The lysosphingolipid sphingosine 1-phosphate (S1P) regulates cell proliferation, apoptosis, motility, and neurite retraction. Its actions may be both intracellular as a second messenger and extracellular as a receptor ligand. S1P and the structurally related lysolipid mediator lysophosphatidic acid (LPA) signal cells through a set of G protein-coupled receptors known as EDG receptors. Some EDG receptors are S1P receptors; others are LPA receptors. According to these guidelines, a receptor is to be named with the abbreviation of the natural agonist with the highest potency, followed by a subscripted arabic number. Thus they suggested that the designation EDG5 should be changed to S1P2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sphingosine 1-phosphate receptor 2
NCBI Official Synonym Full Names
sphingosine-1-phosphate receptor 2
NCBI Official Symbol
S1pr2
NCBI Official Synonym Symbols
Edg5; H218; LPb2; S1P2; Gpcr13; 1100001A16Rik
NCBI Protein Information
sphingosine 1-phosphate receptor 2
UniProt Protein Name
Sphingosine 1-phosphate receptor 2
Protein Family
UniProt Gene Name
S1pr2
UniProt Synonym Gene Names
Edg5; Gpcr13; Lpb2; S1P receptor 2; S1P2; S1P receptor Edg-5
UniProt Entry Name
S1PR2_MOUSE
Uniprot Description
EDG5: Receptor for the lysosphingolipid sphingosine 1- phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; Motility/polarity/chemotaxis; Membrane protein, integral; Lipid-binding; Receptor, GPCR; GPCR, family 1
Cellular Component: integral to membrane; membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; G-protein-coupled receptor binding; integrin binding; signal transducer activity
Biological Process: actin cytoskeleton reorganization; filopodium formation; G-protein coupled receptor protein signaling pathway; signal transduction
Research Articles on S1PR2
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Product Notes
The Mouse S1PR2 s1pr2 (Catalog #AAA9717092) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717092 ELISA Kit recognizes Mouse S1PR2. It is sometimes possible for the material contained within the vial of "Sphingosine 1-Phosphate Receptor 2 (S1PR2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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