Rabbit anti-Mouse Abl1 Polyclonal Antibody | anti-Abl1 antibody

Abl1, ID (Abl1, Abl, Tyrosine-protein kinase ABL1, Abelson murine leukemia viral oncogene homolog 1, Abelson tyrosine-protein kinase 1, Proto-oncogene c-Abl, p150) (APC)

Cat. Num. AAA6270651

• Gene Names: Abl1; Abl; c-Abl; AI325092; E430008G22Rik
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Abl1; Polyclonal Antibody; ID (Abl1; Abl; Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150) (APC); p150; anti-Abl1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Abl1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Abl1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a portion within aa 613-641 from the Central region of human Mouse Abl1 (NP_033724), conjugated to KLH.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Abl1 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Abl1 antibody detected the Abl1 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Abl1 Antibody (Center) MBS6005021 immunohistochemistry analysis in formalin fixed and paraffin embedded mouse duodenum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-Abl1 antibody

Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.

Product Categories/Family for anti-Abl1 antibody

Antibodies; Protein Kinases

References

Ko, H.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(38):16691-16696(2010). Yamaguchi, T., et al. Apoptosis 15(8):927-935(2010). Michael, M., et al. Curr. Biol. 20(9):783-791(2010). Swimm, A.I., et al. J. Virol. 84(9):4243-4251(2010). de Arce, K.P., et al. J. Neurosci. 30(10):3728-3738(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 162951865
• NCBI GeneID: 11350
• NCBI Accession #: NP_033724.2; NP_001106174.1
• NCBI GenBank Nucleotide #: NM_009594.4
• UniProt Accession #: P00520; P97896; Q61252; Q61253; Q61254; Q61255; Q61256; Q61257; Q61258; Q61259; Q61260; Q61261
• Molecular Weight: 124,769 Da
• NCBI Official Full Name: tyrosine-protein kinase ABL1 isoform b
• NCBI Official Synonym Full Names: c-abl oncogene 1, non-receptor tyrosine kinase
• NCBI Official Symbol: Abl1
• NCBI Official Synonym Symbols: Abl; c-Abl; AI325092; E430008G22Rik
• NCBI Protein Information: tyrosine-protein kinase ABL1
• UniProt Protein Name: Tyrosine-protein kinase ABL1
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Abl1
• UniProt Synonym Gene Names: Abl
• UniProt Entry Name: ABL1_MOUSE

Uniprot Description

Function: Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-191' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. Ref.11 Ref.12 Ref.13 Ref.18 Ref.19 Ref.20 Ref.22 Ref.24 Ref.25 Ref.27 Ref.33

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.30 Ref.33 Ref.35

Cofactor: Magnesium or manganese.

Enzyme regulation: Stabilized in the inactive form by an association between the SH3 domain and the SH2-TK linker region, interactions of the N-terminal cap, and contributions from an N-terminal myristoyl group and phospholipids. Activated by autophosphorylation as well as by SRC-family kinase-mediated phosphorylation

By similarity. Activated by RIN1 binding to the SH2 and SH3 domains. Also stimulated by cell death inducers and DNA-damage

By similarity. Phosphatidylinositol 4,5-bisphosphate (PIP2), a highly abundant phosphoinositide known to regulate cytoskeletal and membrane proteins, inhibits also the tyrosine kinase activity. Inhibited by imatinib mesylate (Gleevec). Ref.13 Ref.24 Ref.25 Ref.30 Ref.31 Ref.35

Subunit structure: Interacts with INPPL1/SHIP2. Interacts with SORBS1 following insulin stimulation. Found in a trimolecular complex containing CDK5 and CABLES1. Interacts with CABLES1 and PSTPIP1. Interacts with ZDHHC16. Interacts with the 14-3-3 proteins, YWHAB, YWHAE, YWHAG, YWHAH, SFN AND YWHAZ; the interaction with 14-3-3 proteins requires phosphorylation on Thr-734 and sequesters ABL1 into the cytoplasm. Interacts (via SH3 domain) with CASP9; the interaction is direct and increases in the response of cells to genotoxic stress and ABL1/c-Abl activation

By similarity. Interacts with ABI1, ABI2, BCR, CRK, FYN, LYN, PSMA7 RAD9A, RAD51, RAD52, TP73 and WASF3. A complex made of ABL1, CTTN and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Interacts with STX17; probably phosphorylates STX17

By similarity. Interacts with ITGB1, HCK and FGR. Ref.15 Ref.16 Ref.18 Ref.21 Ref.24 Ref.27

Subcellular location: Cytoplasm › cytoskeleton. Nucleus. Mitochondrion. Note: The myristoylated c-ABL protein is reported to be nuclear. Sequestered into the cytoplasm through interaction with 14-3-3 proteins

By similarity. Localizes to mitochondria in response to oxidative stress. Ref.13 Ref.14 Ref.19

Tissue specificity: Widely expressed.

Post-translational modification: Acetylated at Lys-710 by EP300 which promotes the cytoplasmic translocation

By similarity.Phosphorylation at Tyr-70 by members of the SRC family of kinases disrupts SH3 domain-based autoinhibitory interactions and intermolecular associations, such as that with ABI1, and also enhances kinase activity

By similarity. Phosphorylation at Tyr-226 and Tyr-393 correlate with increased activity

By similarity. DNA damage-induced activation of ABL1 requires the function of ATM and Ser-446 phosphorylation. Phosphorylation at Thr-547 and Ser-569 has been attributed to a CDC2-associated kinase and is coupled to cell division. Phosphorylation at Ser-618 and Ser-619 by PAK2 increases binding to CRK and reduces binding to ABI1

By similarity. Phosphorylation on Thr-734 is required for binding 14-3-3 proteins for cytoplasmic translocation

By similarity. Phosphorylated by PDGFRB and PRKDC. Ref.7 Ref.10 Ref.13 Ref.24 Ref.25 Ref.27 Ref.30 Ref.35Polyubiquitinated. Polyubiquitination of ABL1 leads to degradation

By similarity.Isoform IV is myristoylated on Gly-2.

Disruption phenotype: Mutants are born with the expected Mendelian frequency, but fail to thrive and most die within three weeks after birth. Most mutants are runted, and have atrophied thymuses with severe thymocyte deficiency. Mutants that survive to weaning age are most often runted, and about half of them show lymphopenia. They display a major reduction in the number of pre-B and immature B-cell classes in bone marrow with a wide variation between individuals, but essentially normal mature B-cell levels. Mutants are highly susceptible to infections. Ref.8 Ref.9

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.Contains 1 protein kinase domain.Contains 1 SH2 domain.Contains 1 SH3 domain.