Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transient receptor potential cation channel subfamily M member 4 (TRPM4) Standard Curve.)
Mouse Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4) ELISA Kit | TRPM4 elisa kit
Mouse Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4) ELISA Kit
Gene Names
Trpm4; LTRPC4; TRPM4B; LTrpC-4; AW047689; 1110030C19Rik
Reactivity
Mouse
Synonyms
Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4); Mouse Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4) ELISA Kit; TRPM4; FLJ20041; TRPM4B; TRPM4 elisa kit
Reactivity
Mouse
Specificity
Mouse Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TRPM4. No significant cross-reactivity or interference between Mouse TRPM4 and analogues was observed.
Sequence Length
1213
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transient receptor potential cation channel subfamily M member 4 (TRPM4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transient receptor potential cation channel subfamily M member 4 (TRPM4) Standard Curve.)
Related Product Information for TRPM4 elisa kit
Description: This Mouse Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPM4 in samples. An antibody specific for TRPM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRPM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPM4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPM4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TRPM4 has 1,040 amino acids and contains 6 putative transmembrane domains. Northern blot analysis detected TRPM4 expression in most adult tissues tested, with highest levels in heart, prostate, and colon. In fetus, TRPM4 expression was most abundant in kidney. There were at least 2 distinct bands detected (6.2 and 4.2 kb), indicating alternative splicing of TRPM4. In addition, a smaller but relatively abundant 2.4-kb transcript was detected in testis.The shorter protein results from alternative splicing, with the third and fourth exons being spliced out and the beginning of the first exon being truncated. As a consequence, the first in-frame methionine lies in the fifth exon, resulting in the deletion of the first 174 amino acids.
Background: TRPM4 has 1,040 amino acids and contains 6 putative transmembrane domains. Northern blot analysis detected TRPM4 expression in most adult tissues tested, with highest levels in heart, prostate, and colon. In fetus, TRPM4 expression was most abundant in kidney. There were at least 2 distinct bands detected (6.2 and 4.2 kb), indicating alternative splicing of TRPM4. In addition, a smaller but relatively abundant 2.4-kb transcript was detected in testis.The shorter protein results from alternative splicing, with the third and fourth exons being spliced out and the beginning of the first exon being truncated. As a consequence, the first in-frame methionine lies in the fifth exon, resulting in the deletion of the first 174 amino acids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transient receptor potential cation channel subfamily M member 4
NCBI Official Synonym Full Names
transient receptor potential cation channel, subfamily M, member 4
NCBI Official Symbol
Trpm4
NCBI Official Synonym Symbols
LTRPC4; TRPM4B; LTrpC-4; AW047689; 1110030C19Rik
NCBI Protein Information
transient receptor potential cation channel subfamily M member 4
UniProt Protein Name
Transient receptor potential cation channel subfamily M member 4
Protein Family
UniProt Gene Name
Trpm4
UniProt Synonym Gene Names
Ltrpc4; LTrpC-4; LTrpC4
Uniprot Description
Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca2+, it is impermeable to it. Mediates transport of monovalent cations (Na+ > K+ > Cs+ > Li+), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca2+ oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca2+ overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway (). Essential for the migration but not the maturation of dendritic cells.
Research Articles on TRPM4
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Product Notes
The Mouse TRPM4 trpm4 (Catalog #AAA9716665) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716665 ELISA Kit recognizes Mouse TRPM4. It is sometimes possible for the material contained within the vial of "Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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