Rabbit anti-Human, Mouse CYP3A43 Polyclonal Antibody | anti-CYP3A43 antibody

CYP3A43, ID (CYP3A43, Cytochrome P450 3A43) (FITC)

Cat. Num. AAA6290532

• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunofluorescence, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CYP3A43; Polyclonal Antibody; ID (CYP3A43; Cytochrome P450 3A43) (FITC); Cytochrome P450 3A43; anti-CYP3A43 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP3A43. Species Crossreactivity: mouse
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CYP3A43 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IF: 1:10-50; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CYP3A43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249~278 amino acids from the Center region of human CYP3A43.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of CYP3A43 antibody (Center) in mouse brain tissue lysates (35ug/lane). CYP3A43 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(CYP3A43 Antibody (Center) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CYP3A43 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-CYP3A43 antibody

CYP3A43 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein has a low level of testosterone hydroxylase activity. Although it bears homology to some drug-metabolizing cytochrome P450s, the protein unknown whether the enzyme is also involved in xenobiotic metabolism.

Product Categories/Family for anti-CYP3A43 antibody

Antibodies; Enzymes; Cytochrome

References

Domanski T.L., Finta C.Mol. Pharmacol. 59:386-392(2001). Westlind A., Malmebo S.Biochem. Biophys. Res. Commun. 281:1349-1355(2001). Cauffiez C., Lo-Guidice J.-M.Hum. Mutat. 23:101-101(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 12383060
• NCBI GeneID: 64816
• NCBI Accession #: NP_073731.1
• NCBI GenBank Nucleotide #: NM_022820.3
• UniProt Accession #: Q9HB55; Q75MK2; Q9HB52; Q9HB53; Q9HB54; Q9HB57
• Molecular Weight: 57,670 Da
• NCBI Official Full Name: cytochrome P450 3A43 isoform 1
• NCBI Official Synonym Full Names: cytochrome P450, family 3, subfamily A, polypeptide 43
• NCBI Official Symbol: CYP3A43
• NCBI Protein Information: cytochrome P450 3A43; cytochrome P450 polypeptide 43; cytochrome P450, subfamily IIIA, polypeptide 43
• UniProt Protein Name: Cytochrome P450 3A43
• Protein Family: Cytochrome
• UniProt Gene Name: CYP3A43
• UniProt Entry Name: CP343_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme has a low level of testosterone hydroxylase activity. Although it bears homology to some drug-metabolizing cytochrome P450s, it is unknown whether the enzyme is also involved in xenobiotic metabolism. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Alternate splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP3A43: Exhibits low testosterone 6-beta-hydroxylase activity. Belongs to the cytochrome P450 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.14.1; Oxidoreductase; Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: endoplasmic reticulum membrane

Molecular Function: iron ion binding; heme binding; monooxygenase activity

Biological Process: xenobiotic metabolic process