Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphoribosyltransferase domain-containing protein 1 (PRTFDC1) Standard Curve.)
Human Phosphoribosyltransferase Domain-Containing Protein 1 (PRTFDC1) ELISA Kit | PRTFDC1 elisa kit
Human Phosphoribosyltransferase Domain-Containing Protein 1 (PRTFDC1) ELISA Kit
Gene Names
PRTFDC1; HHGP
Reactivity
Human
Synonyms
Phosphoribosyltransferase Domain-Containing Protein 1 (PRTFDC1); Human Phosphoribosyltransferase Domain-Containing Protein 1 (PRTFDC1) ELISA Kit; PRTFDC1; FLJ11888; HHGP; PRTFDC1 elisa kit
Reactivity
Human
Specificity
Human Phosphoribosyltransferase domain-containing protein 1 (PRTFDC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRTFDC1. No significant cross-reactivity or interference between Human PRTFDC1 and analogues was observed.
Sequence Length
190
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphoribosyltransferase domain-containing protein 1 (PRTFDC1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphoribosyltransferase domain-containing protein 1 (PRTFDC1) Standard Curve.)
Related Product Information for PRTFDC1 elisa kit
Description: This Human Phosphoribosyltransferase domain-containing protein 1 (PRTFDC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRTFDC1 in samples. An antibody specific for PRTFDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRTFDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRTFDC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRTFDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The predicted PRTFDC1 protein contains a phosphoribosyl transferase domain, but it lacks conservation of 11 HPRT1 residues critical for HPRT function, suggesting that PRTFDC1 has lost its ancestral HPRT activity. Database analysis revealed that Prtfdc1 is present in several vertebrate species. However, in mouse, the Prtfdc1 gene appeared to be inactive due to 3 independent mutations. PRTFDC1was inactivated either by deletion or hypermethylation in a significant subset of oral squamous cell carcinomas. Restoration of PRTFDC1 expression in 1 of these lines inhibited cell growth in colony formation assays, whereas knockdown of PRTFDC1 expression in PRTFDC1-expressing cells promoted cell growth.
Background: The predicted PRTFDC1 protein contains a phosphoribosyl transferase domain, but it lacks conservation of 11 HPRT1 residues critical for HPRT function, suggesting that PRTFDC1 has lost its ancestral HPRT activity. Database analysis revealed that Prtfdc1 is present in several vertebrate species. However, in mouse, the Prtfdc1 gene appeared to be inactive due to 3 independent mutations. PRTFDC1was inactivated either by deletion or hypermethylation in a significant subset of oral squamous cell carcinomas. Restoration of PRTFDC1 expression in 1 of these lines inhibited cell growth in colony formation assays, whereas knockdown of PRTFDC1 expression in PRTFDC1-expressing cells promoted cell growth.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphoribosyltransferase domain-containing protein 1 isoform 2
NCBI Official Synonym Full Names
phosphoribosyl transferase domain containing 1
NCBI Official Symbol
PRTFDC1
NCBI Official Synonym Symbols
HHGP
NCBI Protein Information
phosphoribosyltransferase domain-containing protein 1
UniProt Protein Name
Phosphoribosyltransferase domain-containing protein 1
Protein Family
UniProt Gene Name
PRTFDC1
UniProt Synonym Gene Names
HHGP
UniProt Entry Name
PRDC1_HUMAN
Uniprot Description
PRTFDC1: Has low, barely detectable phosphoribosyltransferase activity (in vitro). Binds GMP, IMP and alpha-D-5-phosphoribosyl 1-pyrophosphate (PRPP). Is not expected to contribute to purine metabolism or GMP salvage. Belongs to the purine/pyrimidine phosphoribosyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 10p12.1
Cellular Component: cytosol
Molecular Function: protein homodimerization activity; nucleotide binding; hypoxanthine phosphoribosyltransferase activity; magnesium ion binding
Biological Process: GMP catabolic process; hypoxanthine metabolic process; IMP salvage; GMP salvage; adenine salvage; purine ribonucleoside salvage; guanine salvage
Research Articles on PRTFDC1
Similar Products
Product Notes
The Human PRTFDC1 prtfdc1 (Catalog #AAA9714765) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714765 ELISA Kit recognizes Human PRTFDC1. It is sometimes possible for the material contained within the vial of "Phosphoribosyltransferase Domain-Containing Protein 1 (PRTFDC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
