Rabbit anti-Human, Mouse DENND1B Polyclonal Antibody | anti-DENND1B antibody
DENND1B Antibody
Gene Names
DENND1B; FAM31B; C1ORF18; C1orf218
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
DENND1B; Polyclonal Antibody; DENND1B Antibody; C1ORF18; C1orf218; Chromosome 1 open reading frame 218; Connecdenn 2; DEN1B_HUMAN; DENN domain containing protein 1B; DENN domain-containing protein 1B; DENN MADD domain containing 1B; DKFZp547O0715; FAM31B; Family with sequence similarity 31 member B; FLJ20054; FLJ21179; FLJ33888; MGC103810; MGC27044; Protein FAM31B; RP11 53I24.2; anti-DENND1B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
DENND1B Antibody detects endogenous levels of DENND1B.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-DENND1B antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human DENND1B.
Conjugation
Unconjugated
Similarity
The FXDXF motif mediates interaction the AP-2 complex. The clathrin box motif mediates interaction with clathrin.
Post Translational Modifications
Phosphorylated on serine and/or threonine, possibly regulating the guanine nucleotide exchange factor (GEF) activity.
Subunit Structure
Interacts with RAB35. Interacts with clathrin heavy chain/CLTC. Interacts with components of the adapter protein complex 2 (AP-2) AP2A2 and AP2B1. Interacts with CD3E (By similarity).
Tissue Specificity
Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T-cells as compared with naive T-cells.
Function
Guanine nucleotide exchange factor (GEF) for RAB35 that acts as a regulator of T-cell receptor (TCR) internalization in TH2 cells. Acts by promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Plays a role in clathrin-mediated endocytosis. Controls cytokine production in TH2 lymphocytes by controlling the rate of TCR internalization and routing to endosomes: acts by mediating clathrin-mediated endocytosis of TCR via its interaction with the adapter protein complex 2 (AP-2) and GEF activity. Dysregulation leads to impaired TCR down-modulation and recycling, affecting cytokine production in TH2 cells.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Product Categories/Family for anti-DENND1B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
86,552 Da
NCBI Official Full Name
DENN domain-containing protein 1B isoform 3
NCBI Official Synonym Full Names
DENN/MADD domain containing 1B
NCBI Official Symbol
DENND1B
NCBI Official Synonym Symbols
FAM31B; C1ORF18; C1orf218
NCBI Protein Information
DENN domain-containing protein 1B; connecdenn 2; family with sequence similarity 31, member B
UniProt Protein Name
DENN domain-containing protein 1B
Protein Family
UniProt Gene Name
DENND1B
UniProt Synonym Gene Names
C1orf218; FAM31B
UniProt Entry Name
DEN1B_HUMAN
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Product Notes
The DENND1B dennd1b (Catalog #AAA9631177) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DENND1B Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DENND1B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the DENND1B dennd1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DENND1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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