Rabbit anti-Human DENND1B Polyclonal Antibody | anti-DENND1B antibody

DENND1B antibody

Cat. Num. AAA9408271

• Gene Names: DENND1B; FAM31B; C1ORF18; C1orf218
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: DENND1B; Polyclonal Antibody; DENND1B antibody; anti-DENND1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 426

• Applicable Applications for anti-DENND1B antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500-1:3000; Immunofluorescence: 1:100-1:200
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 242 of DENND1B
• Target Name: DENND1B
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate)A: MOLT4 7.5% SDS PAGEPrimary antibody diluted at 1: 1000)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DENND1B antibody at 1: 200 dilution.)

Product Categories/Family for anti-DENND1B antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 188219541
• NCBI GeneID: 163486
• NCBI Accession #: NP_659414
• NCBI GenBank Nucleotide #: NM_144977.4
• Molecular Weight: 86,552 Da
• NCBI Official Full Name: DENN domain-containing protein 1B isoform 2
• NCBI Official Synonym Full Names: DENN/MADD domain containing 1B
• NCBI Official Symbol: DENND1B
• NCBI Official Synonym Symbols: FAM31B; C1ORF18; C1orf218
• NCBI Protein Information: DENN domain-containing protein 1B
• UniProt Protein Name: DENN domain-containing protein 1B
• Protein Family: DENN domain-containing protein
• UniProt Gene Name: DENND1B
• UniProt Synonym Gene Names: C1orf218; FAM31B
• UniProt Entry Name: DEN1B_HUMAN

NCBI Description

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]

Uniprot Description

DENND1B: a guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Interacts with RAB35. Interacts with clathrin heavy chain/CLTC and with AP2A2. Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T-cells as compared with naive T-cells. Five isoforms of the human protein are produced by alternative splicing. Isoform 5 interacts with AP2B1.

Protein type: GEFs, Rab; GEFs

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: clathrin-coated vesicle; cytosol

Molecular Function: Rab guanyl-nucleotide exchange factor activity

Biological Process: positive regulation of GTPase activity; protein transport