Mouse anti-Human CD1a Monoclonal Antibody | anti-CD1a antibody

anti-human CD1a-FITC

Cat. Num. AAA666201

• Gene Names: CD1A; R4; T6; CD1; FCB6; HTA1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD1a; Monoclonal Antibody; anti-human CD1a-FITC; Monoclonal anti-human CD1a/FITC; anti-CD1a antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: CB-T6
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Sequence Length: 327

• Applicable Applications for anti-CD1a antibody: Flow Cytometry (FC/FACS)
• Application Notes: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)
• Performance: Five x 105 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD1a/FITC at a 1:50 dilution (10 ug/ml). Cells were washed three times, fixed and analyzed by FACS Calibur. Cells stained positive with a mean shift of 0.87 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD1a antibody.
• Conjugation: FITC
• Production: Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.5 mg/ml with a Fluorescein:Ab Molar ratio of 10.2.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Human Jurkat tumor cells
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD1a antibody

Information: The human CD1 genetic locus consists of five closely related genes (1). Three of the gene products (CD1a, CD1b and CD1c) have been officially clustered using antibodies and are expressed on cortical thymocytes, a subset of B cells, and on most professional antigen presenting cells (2). CD1 molecules have a specialized lipid antigen-presenting function (3,4) and it is unlikely that they present conventional peptide antigens. CD1a is an important marker to help identify developmental pathways of dendritic cells (5).

References

(1) Leukocyte Typing III (A.J. McMichael, et al, eds.) Oxford University Press, Oxford, (1987) p. 882. (2) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 337-341. (3) E.M. Beckman, et al, (1994). (4) Nature 372: 691-694. R.S. Blumberg, et al, (1995) Immunol Rev 147: 5-29. (5) T. Ito, et al, (1999) J Immunol 163: 1409-1419.

NCBI and Uniprot Product Information

• NCBI GI #: 180036
• NCBI GeneID: 909
• Molecular Weight: 37,077 Da
• NCBI Official Full Name: CD1a antigen
• NCBI Official Synonym Full Names: CD1a molecule
• NCBI Official Symbol: CD1A
• NCBI Official Synonym Symbols: R4; T6; CD1; FCB6; HTA1
• NCBI Protein Information: T-cell surface glycoprotein CD1a; hTa1 thymocyte antigen; CD1A antigen, a polypeptide; cluster of differentiation 1 A; T-cell surface antigen T6/Leu-6; cortical thymocyte antigen CD1A; differentiation antigen CD1-alpha-3; epidermal dendritic cell marker CD1a
• UniProt Protein Name: T-cell surface glycoprotein CD1a
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD1A
• UniProt Synonym Gene Names: hTa1 thymocyte antigen
• UniProt Entry Name: CD1A_HUMAN

NCBI Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CD1A: Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: integral to plasma membrane; plasma membrane; endosome membrane

Molecular Function: protein binding; beta-2-microglobulin binding; exogenous lipid antigen binding; endogenous lipid antigen binding

Biological Process: antigen processing and presentation, exogenous lipid antigen via MHC class Ib; immune response