Western Blot (WB)
(Western blot analysis of extracts from Mouse brain tissue, using PI4KA Antibody. The lane on the left was treated with blocking peptide.)
Rabbit PI4KA Polyclonal Antibody | anti-PI4KA antibody
PI4KA Antibody
Gene Names
PI4KA; PIK4CA; PMGYCHA; pi4K230; PI4K-ALPHA
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
PI4KA; Polyclonal Antibody; PI4KA Antibody; EC 2.7.1.67; Phosphatidylinositol 4 kinase alpha; Phosphatidylinositol 4 kinase catalytic alpha polypeptide; Phosphatidylinositol 4-kinase alpha; PI4 kinase; PI4-kinase alpha; PI4K ALPHA; PI4K-alpha; pi4K230; PI4KA_HUMAN; PIK4; PIK4CA; PtdIns 4 kinase; PtdIns-4-kinase alpha; anti-PI4KA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
PI4KA Antibody detects endogenous levels of PI4KA.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
2102
Applicable Applications for anti-PI4KA antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human PI4KA.
Tissue Specificity
Expressed ubiquitously. Highest levels in placenta and brain. Little or no expression in lung, liver, pancreas, testis or leukocytes.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Plasma Membrane
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Mouse brain tissue, using PI4KA Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse brain tissue, using PI4KA Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-PI4KA antibody
Description: Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.
Subunit Structure: Component of a phosphatidylinositol 4-kinase (PI4K) complex, composed of PI4KA, EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and FAM126 (FAM126A or FAM126B) (PubMed:23229899, PubMed:24417819, PubMed:26571211). Interacts with TMEM150A; regulating recruitment to the plasma membrane (PubMed:25608530).
Similarity: Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.
Subunit Structure: Component of a phosphatidylinositol 4-kinase (PI4K) complex, composed of PI4KA, EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and FAM126 (FAM126A or FAM126B) (PubMed:23229899, PubMed:24417819, PubMed:26571211). Interacts with TMEM150A; regulating recruitment to the plasma membrane (PubMed:25608530).
Similarity: Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 231 kDa
Predicted: 237 kDa
Predicted: 237 kDa
NCBI Official Full Name
phosphatidylinositol 4-kinase alpha isoform 1
NCBI Official Synonym Full Names
phosphatidylinositol 4-kinase alpha
NCBI Official Symbol
PI4KA
NCBI Official Synonym Symbols
PIK4CA; PMGYCHA; pi4K230; PI4K-ALPHA
NCBI Protein Information
phosphatidylinositol 4-kinase alpha
UniProt Protein Name
Phosphatidylinositol 4-kinase alpha
Protein Family
UniProt Gene Name
PI4KA
UniProt Synonym Gene Names
PIK4; PIK4CA; PI4-kinase alpha; PI4K-alpha; PtdIns-4-kinase alpha
UniProt Entry Name
PI4KA_HUMAN
NCBI Description
This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by this gene is a type III enzyme that is not inhibited by adenosine. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2018]
Research Articles on PI4KA
Similar Products
Product Notes
The PI4KA pi4ka (Catalog #AAA9611447) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PI4KA Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's PI4KA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PI4KA pi4ka for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI4KA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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