Immunohistochemistry (IHC)
(MBS9606895 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit C19orf10 Polyclonal Antibody | anti-C19orf10 antibody
C19orf10 Antibody
Gene Names
MYDGF; IL25; IL27; SF20; IL27w; C19orf10; R33729_1; EUROIMAGE1875335
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
C19orf10; Polyclonal Antibody; C19orf10 Antibody; Chromosome 19 open reading frame 10; CS010_HUMAN; EUROIMAGE1875335; IL 25; IL 27; IL-25; IL25; IL27; IL27w; Interleukin 25; Interleukin 27 working designation; Interleukin-25; Interleukin25; R33729 1; R337291; SF20; Stromal cell derived growth factor; Stromal cell derived growth factor SF20; Stromal cell-derived growth factor SF20; Uncharacterized protein C19orf10 precursor; UPF0556 protein C19orf10; anti-C19orf10 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
C19orf10 antibody detects endogenous levels of total C19orf10
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
173
Applicable Applications for anti-C19orf10 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human C19orf10
Subcellular Location
Secreted. Endoplasmic reticulum-Golgi intermediate compartment.
Tissue Specificity
Expressed in bone marrow cells (PubMed:25581518). Expressed in synovial tissue. Found in synovial fluid of patients with arthropaties (PubMed:17362502).
Predicted Cross Reactivity
Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
Similarity
Zebrafish (92%), Bovine (92%), Horse (92%), Sheep (92%), Dog (92%), Chicken (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9606895 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9606895 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of K562 using C19orf10 antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of K562 using C19orf10 antibody. The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-C19orf10 antibody
Description: The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was considered an interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. [provided by RefSeq, Jul 2008]
Function: Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3-and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (By similarity). Involved in endothelial cell proliferation and angiogenesis (PubMed:25581518).
Similarity: Belongs to the MYDGF family.
Function: Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3-and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (By similarity). Involved in endothelial cell proliferation and angiogenesis (PubMed:25581518).
Similarity: Belongs to the MYDGF family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 19 kDa
Predicted: 19 kDa
Predicted: 19 kDa
NCBI Official Full Name
myeloid-derived growth factor
NCBI Official Synonym Full Names
myeloid derived growth factor
NCBI Official Symbol
MYDGF
NCBI Official Synonym Symbols
IL25; IL27; SF20; IL27w; C19orf10; R33729_1; EUROIMAGE1875335
NCBI Protein Information
myeloid-derived growth factor
UniProt Protein Name
Myeloid-derived growth factor
UniProt Gene Name
MYDGF
UniProt Synonym Gene Names
C19orf10ImportedManual assertion inferred from database entriesiHGNC:16948; IL251 PublicationManual assertion based on opinion iniRef.1; MYDGF1 PublicationManual assertion based on opinion iniRef.10; IL-251 PublicationManual assertion based on opinion ini
NCBI Description
The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was considered an interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. [provided by RefSeq, Jul 2008]
Uniprot Description
Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (). Involved in endothelial cell proliferation and angiogenesis (PubMed:25581518).
Research Articles on C19orf10
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Product Notes
The C19orf10 mydgf (Catalog #AAA9606895) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C19orf10 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's C19orf10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the C19orf10 mydgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C19orf10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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