CD84 recombinant protein

Recombinant Mouse CD84 Protein (His tag)

Cat. Num. AAA2546802

• Gene Names: Cd84; CDw84; SLAMF5; A130013D22Rik
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: CD84; Recombinant Mouse CD84 Protein (His tag); A130013D22Rik; CDw84; SLAMF5; CD84 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 140

• Application Notes: The recombinant mouse CD84 consists of 211 amino acids and has a predicted molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmCD84 is approximately 37-42 kDa due to glycosylation.
• Predicted N Terminal: Lys 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD84 recombinant protein

Background: The CD2 family receptors are type I transmembrane glycoproteins belonging to immunoglobulin (Ig) superfamily characterized by a membrane-proximal Ig constant 2 (C2) domain and a membrane-distal variable (V) domain that is responsible for ligand recognition. CD84, also known as LY9B and SLAMF5, is a homophilic member of the SLAM (signaling lymphocyte activation molecule) subfamily of the CD2 family. The SLAM family receptorsmediate signal transduction through the interaction of its ITSM (immunoreceptor tyrosine-based switch motifs) in the intracellular region and the SH2 domain of adaptor molecules SAP (SLAM-associated protein) and EAT-2 (EWS-activated transcript 2), and accordingly modulate both adaptive and innate immune responses. The CD84-CD84 interaction was independent of its cytoplasmic tail. Thus, CD84 is its own ligand and acts as a costimulatory molecule. CD84 is expressed on cells from almost all hematopoietic lineages and on CD34+ hematopoietic progenitor cells, suggesting that CD84 serves as a marker for committed hematopoietic progenitor cells.

Description: A DNA sequence encoding the mouse CD84 isoform 1 (Q18PI6-1) extracellular domain (Met 1-Val 221) was expressed, with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 357197147
• NCBI GeneID: 12523
• NCBI Accession #: NP_001239401.1
• NCBI GenBank Nucleotide #: NM_001252472.1
• UniProt Accession #: Q18PI6; Q18PI7; Q3TBT1; Q3U0A2; Q8BFV0; Q9Z178
• Molecular Weight: 37,307 Da
• NCBI Official Full Name: SLAM family member 5 isoform 2
• NCBI Official Synonym Full Names: CD84 antigen
• NCBI Official Symbol: Cd84
• NCBI Official Synonym Symbols: CDw84; SLAMF5; A130013D22Rik
• NCBI Protein Information: SLAM family member 5
• UniProt Protein Name: SLAM family member 5
• Protein Family: SLAM family
• UniProt Gene Name: Cd84
• UniProt Synonym Gene Names: Slamf5

Uniprot Description

CD84: Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain- containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Immunoglobulin superfamily; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1 H3|1 79.54 cM

Biological Process: activation of NF-kappaB transcription factor; negative regulation of granulocyte macrophage colony-stimulating factor production; negative regulation of interleukin-18 production; negative regulation of mast cell activation; negative regulation of mast cell degranulation; positive regulation of MAPKKK cascade; regulation of lipopolysaccharide-mediated signaling pathway; regulation of macrophage activation