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Western Blot (WB) (Western blotAll lanes: MYDGF antibody at 9ug/ml+MCF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 19kDaObserved band size: 19kDa)

Rabbit anti-Human MYDGF Polyclonal Antibody | anti-MYDGF antibody

MYDGF Antibody

Gene Names
MYDGF; IL25; IL27; SF20; IL27w; C19orf10; R33729_1; EUROIMAGE1875335
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
MYDGF; Polyclonal Antibody; MYDGF Antibody; Interleukin-25; IL-25; Stromal cell-derived growth factor SF201; C19orf10; IL25; anti-MYDGF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
173
Applicable Applications for anti-MYDGF antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:200-1000
IHC:1:20-1:200
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Myeloid-derived growth factor protein (32-173aa)
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: MYDGF antibody at 9ug/ml+MCF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 19kDaObserved band size: 19kDa)

Western Blot (WB) (Western blotAll lanes: MYDGF antibody at 9ug/ml+MCF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 19kDaObserved band size: 19kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human placenta tissue using MBS1499362 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta tissue using MBS1499362 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human pancreas tissueMBS1499362 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human pancreas tissueMBS1499362 at dilution 1:100)
Related Product Information for anti-MYDGF antibody
Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (By similarity). Involved in endothelial cell proliferation and angiogenesis (PubMed:25581518).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,795 Da
NCBI Official Full Name
myeloid-derived growth factor
NCBI Official Synonym Full Names
myeloid-derived growth factor
NCBI Official Symbol
MYDGF
NCBI Official Synonym Symbols
IL25; IL27; SF20; IL27w; C19orf10; R33729_1; EUROIMAGE1875335
NCBI Protein Information
myeloid-derived growth factor
UniProt Protein Name
Myeloid-derived growth factor
UniProt Gene Name
MYDGF
UniProt Synonym Gene Names
IL-25
UniProt Entry Name
MYDGF_HUMAN

NCBI Description

The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was considered an interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. [provided by RefSeq, Jul 2008]

Uniprot Description

MYDGF: was previously thought to support proliferation of lymphoid cells and was considered an interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: endoplasmic reticulum lumen; ER-Golgi intermediate compartment; extracellular space

Molecular Function: protein binding

Biological Process: angiogenesis; apoptosis; cellular protein metabolic process; negative regulation of apoptosis; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of MAPKKK cascade; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of transcription from RNA polymerase II promoter; unfolded protein response

Research Articles on MYDGF

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Product Notes

The MYDGF mydgf (Catalog #AAA1499362) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYDGF Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYDGF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:200-1000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the MYDGF mydgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYDGF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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