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SDS-Page

SF20/IL25 recombinant protein

SF20/IL25, 33-173aa, Human, His tag, E Coli

Gene Names
MYDGF; IL25; IL27; SF20; IL27w; C19orf10; R33729_1; EUROIMAGE1875335
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
SF20/IL25; 33-173aa; Human; His tag; E Coli; Stromal cell-derived growth factor; C19orf10; IL25; uPF0556; IL27; IL27w; Stromal cell-derived growth factor Chromosome 19 open reading frame 10; EuROIMAGE1875335; IL 25; IL 27; Interleukin 25; Interleukin 27 working designation; Interleukin25; R33729 1; R337291; SF20; Stromal cell derived growth factor; Stromal cell derived growth factor SF20; uncharacterized protein C19orf10 precursor.; SF20/IL25 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MSEPTTVAFD VRPGGVVHSF SHNVGPGDKY TCMFTYASQG GTNEQWQMSL GTSEDHQHFT CTIWRPQGKS YLYFTQFKAE VRGAEIEYAM AYSKAAFERE SDVPLKTEEF EVTKTAVAHR PGAFKAELSK LVIVAKASRT EL
Sequence Length
173
Applicable Applications for SF20/IL25 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for SF20/IL25 recombinant protein
SF20/25, originally identified as a product of bone marrow-derived stromal cells, was previously thought to support proliferation of lymphoid cells and was designated as interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. Recombinant human SF20/IL25 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Categories/Family for SF20/IL25 recombinant protein
References
Weiler T., et al. (2007) Arthritis Res Ther. 9, R30.; Tulin E.E., et al. (2001) J. Immunol.167 (11):6338-47;

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18 kDa (162 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
myeloid-derived growth factor
NCBI Official Synonym Full Names
myeloid derived growth factor
NCBI Official Symbol
MYDGF
NCBI Official Synonym Symbols
IL25; IL27; SF20; IL27w; C19orf10; R33729_1; EUROIMAGE1875335
NCBI Protein Information
myeloid-derived growth factor
UniProt Protein Name
Myeloid-derived growth factor
UniProt Gene Name
MYDGF
UniProt Synonym Gene Names
IL-25

NCBI Description

The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was considered an interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. [provided by RefSeq, Jul 2008]

Uniprot Description

Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (). Involved in endothelial cell proliferation and angiogenesis (PubMed:25581518).

Research Articles on SF20/IL25

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Product Notes

The SF20/IL25 mydgf (Catalog #AAA203227) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's SF20/IL25 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SF20/IL25 mydgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MSEPTTVAFD VRPGGVVHSF SHNVGPGDKY TCMFTYASQG GTNEQWQMSL GTSEDHQHFT CTIWRPQGKS YLYFTQFKAE VRGAEIEYAM AYSKAAFERE SDVPLKTEEF EVTKTAVAHR PGAFKAELSK LVIVAKASRT EL. It is sometimes possible for the material contained within the vial of "SF20/IL25, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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