Testing Data
(Detection of Recombinant Mouse Interferon Gamma in Sandwich ELISA using Rat anti Mouse Interferon Gamma: Endotoxin Low (MBS216933) as a coating antibody and a biotinylated Rabbit anti Mouse Interferon Gamma as the detection reagent)
Rat IFN GAMMA Monoclonal Antibody | anti-Ifng antibody
RAT ANTI MOUSE INTERFERON GAMMA
Purified IgG - liquid
ELISA: Minimum Dilution: 1ug/ml; Maximum Dilution: 10ug/ml
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50; Application Note: Membrane permeablisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Shelf Life: 18 months from date of despatch.
Testing Data
(Detection of Recombinant Mouse Interferon Gamma in Sandwich ELISA using Rat anti Mouse Interferon Gamma: Endotoxin Low (MBS216933) as a coating antibody and a biotinylated Rabbit anti Mouse Interferon Gamma as the detection reagent)
Testing Data
(Detection of Recombinant Mouse Interferon Gamma in Sandwich ELISA using Rat anti Mouse Interferon Gamma: Endotoxin Low (MBS216933) as a coating antibody and a biotinylated Rabbit anti Mouse Interferon Gamma as the detection reagent)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]
Uniprot Description
IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.
Protein type: Membrane protein, integral; Cytokine; Secreted, signal peptide; Secreted
Cellular Component: extracellular space; cell; extracellular region; intracellular; external side of plasma membrane
Molecular Function: protein binding; interferon-gamma receptor binding; cytokine activity
Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of cell adhesion; positive regulation of osteoclast differentiation; positive regulation of interleukin-23 production; positive regulation of transcription, DNA-dependent; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; positive regulation of killing of cells of another organism; positive regulation of membrane protein ectodomain proteolysis; regulation of growth; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; neutrophil chemotaxis; positive regulation of synaptic transmission, cholinergic; CD8-positive, alpha-beta T cell differentiation during immune response; negative regulation of myelination; response to virus; defense response to protozoan; positive regulation of tumor necrosis factor production; inflammatory cell apoptosis; positive regulation of tyrosine phosphorylation of Stat1 protein; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; apoptosis; positive regulation of interleukin-12 production; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-6 biosynthetic process; sensory perception of mechanical stimulus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; regulation of immune response; adaptive immune response; unfolded protein response; humoral immune response; antigen processing and presentation; positive regulation of chemokine biosynthetic process; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; protein import into nucleus, translocation; immune response; positive regulation of neuron differentiation; regulation of insulin secretion
Research Articles on Ifng
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Product Notes
The Ifng ifng (Catalog #AAA212196) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IFN GAMMA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow cytometry (FC/FACS)*. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ELISA: Minimum Dilution: 1ug/ml; Maximum Dilution: 10ug/ml Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50; Application Note: Membrane permeablisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. Researchers should empirically determine the suitability of the Ifng ifng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFN GAMMA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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