Rabbit MANF Polyclonal Antibody | anti-MANF antibody

MANF Conjugated Antibody

Cat. Num. AAA9447788

• Gene Names: NUAK1; ARK5
• Reactivity: Human, Mouse, Rat
• Applications: Most Applications
• Synonyms: MANF; Polyclonal Antibody; MANF Conjugated Antibody; ARP; ARMET; anti-MANF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total MANF protein.
• Form/Format: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide

• Applicable Applications for anti-MANF antibody: Most Applications
• Application Notes: Suggested Dilution:; AF350 conjugated: most applications: 1:50-1:250; AF405 conjugated: most applications: 1:50-1:250; AF488 conjugated: most applications: 1:50-1:250; AF555 conjugated: most applications: 1:50-1:250; AF594 conjugated: most applications: 1:50-1:250; AF647 conjugated: most applications: 1:50-1:250; AF680 conjugated: most applications: 1:50-1:250; AF750 conjugated: most applications: 1:50-1:250; Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000
• Immunogen: Synthetic peptide corresponding to residues near the C terminal of human mesencephalic astrocyte-derived neurotrophic factor
• Conjugation: Biotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation Emission: AF350: 346nm/442nm
• Preparation and Storage: Store at 4 degree C for 6 months.

Related Product Information for anti-MANF antibody

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of polymorphisms in the arginine-rich region, including a specific mutation that changes the previously numbered codon 50 from ATG to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus not tumor-related.

NCBI and Uniprot Product Information

• NCBI GI #: 7662170
• NCBI GeneID: 9891
• NCBI Accession #: NP_055655
• NCBI GenBank Nucleotide #: NM_014840
• UniProt Accession #: P55145; Q96KA8; A7MD39; O60285
• Molecular Weight: 23,824 Da
• NCBI Official Full Name: NUAK family SNF1-like kinase 1
• NCBI Official Synonym Full Names: NUAK family, SNF1-like kinase, 1
• NCBI Official Symbol: NUAK1
• NCBI Official Synonym Symbols: ARK5
• NCBI Protein Information: NUAK family SNF1-like kinase 1; AMP-activated protein kinase family member 5; AMPK-related protein kinase 5; omphalocele kinase 1
• UniProt Protein Name: Mesencephalic astrocyte-derived neurotrophic factor
• Protein Family: MANF/CDNF-like protein
• UniProt Gene Name: MANF
• UniProt Synonym Gene Names: ARMET; ARP
• UniProt Entry Name: MANF_HUMAN

Uniprot Description

ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular space; perinuclear region of cytoplasm; endoplasmic reticulum; nucleus

Molecular Function: growth factor activity

Biological Process: vasoconstriction of artery involved in ischemic response to lowering of systemic arterial blood pressure; response to unfolded protein

Disease: Pancreatic Cancer