Rabbit anti-Human ARMET Polyclonal Antibody | anti-MANF antibody

ARMET, CT (Arginine-rich Mutated in Early Stage Tumors, Arginine-rich Protein, ARP, Mesencephalic Astrocyte-derived Neurotrophic Factor, MANF, MGC142148, MGC142150)

Cat. Num. AAA646245

• Gene Names: MANF; ARP; ARMET
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ARMET; Polyclonal Antibody; CT (Arginine-rich Mutated in Early Stage Tumors; Arginine-rich Protein; ARP; Mesencephalic Astrocyte-derived Neurotrophic Factor; MANF; MGC142148; MGC142150); Anti -ARMET; anti-MANF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human ARMET. Species sequence homology: mouse and rat
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-MANF antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 12aa from near the C-terminal of human MANF.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with ARMET antibody at 5ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

(Western blot analysis of MANF in mouse brain tissue lysate with MANF antibody at (A) 1 and (B) 2ug/ml. Below: Immunohistochemistry of MANF in human brain tissue with MANF antibody at 2.5ug/ml.)

Related Product Information for anti-MANF antibody

Armet encodes a highly conserved protein whose function is not yet known. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors.

Product Categories/Family for anti-MANF antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 13938307
• NCBI GeneID: 7873
• UniProt Accession #: P55145; Q14CX4; Q86U67; Q96IS4
• Molecular Weight: 20,700 Da
• NCBI Official Full Name: ARMET protein, partial
• NCBI Official Synonym Full Names: mesencephalic astrocyte-derived neurotrophic factor
• NCBI Official Symbol: MANF
• NCBI Official Synonym Symbols: ARP; ARMET
• NCBI Protein Information: mesencephalic astrocyte-derived neurotrophic factor; arginine-rich, mutated in early stage tumors
• UniProt Protein Name: Mesencephalic astrocyte-derived neurotrophic factor
• Protein Family: Alpha-mannosidase
• UniProt Gene Name: MANF
• UniProt Synonym Gene Names: ARMET; ARP
• UniProt Entry Name: MANF_HUMAN

NCBI Description

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of polymorphisms in the arginine-rich region, including a specific mutation that changes the previously numbered codon 50 from ATG to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus not tumor-related. [provided by RefSeq, Jun 2010]

Uniprot Description

Function: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons

By similarity. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Ref.6 Ref.8

Subcellular location: Secreted Ref.6 Ref.8.

Induction: By endoplasmic reticulum stress. Ref.8

Domain: The N-terminal region may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. Ref.10

Post-translational modification: May contain sialic acid residues.

Sequence similarities: Belongs to the ARMET family.

Caution: Was originally (Ref.1, Ref.11 and Ref.12) thought to be much longer and included an arginine-rich region thought to be the target of cancer-causing mutations. All these mutations are in what is now thought to be the 5'-UTR of the mRNA.It is uncertain whether Met-1 or Met-4 is the initiator.

Sequence caution: The sequence AAB08753.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence AAI13589.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence AAI13591.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.