Western Blot (WB)
(Western Blot analysis of MCF-7 cells using CHD4 Monoclonal Antibody at dilution of 1:1000)
Mouse anti-Human CHD4 Monoclonal Antibody | anti-CHD4 antibody
CHD4 Monoclonal Antibody
Gene Names
CHD4; CHD-4; Mi-2b; SIHIWES; Mi2-BETA
Reactivity
Human
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Protein G purification
Synonyms
CHD4; Monoclonal Antibody; CHD4 Monoclonal Antibody; ATP dependent helicase CHD4; ATP-dependent helicase CHD4; CHD 4; CHD-4; Chromodomain helicase DNA binding protein 4; Chromodomain-helicase-DNA-binding protein 4; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi-2 autoantigen 218 kDa protein; Mi2 beta; Mi2-beta; anti-CHD4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2258
Purity/Purification
Protein G purification
Concentration
1mg/mL (varies by lot)
Sequence Length
1912
Applicable Applications for anti-CHD4 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:5000
IF: 1:50-1:500
IF: 1:50-1:500
Immunogen
Fusion protein of CHD4
Buffer
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of MCF-7 cells using CHD4 Monoclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of MCF-7 cells using CHD4 Monoclonal Antibody at dilution of 1:1000)
Related Product Information for anti-CHD4 antibody
Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity.
Product Categories/Family for anti-CHD4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 221kDa
Observed: 260kDa
Observed: 260kDa
NCBI Official Full Name
chromodomain-helicase-DNA-binding protein 4 isoform 1
NCBI Official Synonym Full Names
chromodomain helicase DNA binding protein 4
NCBI Official Symbol
CHD4
NCBI Official Synonym Symbols
CHD-4; Mi-2b; SIHIWES; Mi2-BETA
NCBI Protein Information
chromodomain-helicase-DNA-binding protein 4
UniProt Protein Name
Chromodomain-helicase-DNA-binding protein 4
Protein Family
UniProt Gene Name
CHD4
UniProt Synonym Gene Names
CHD-4
NCBI Description
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
Uniprot Description
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
Research Articles on CHD4
Similar Products
Product Notes
The CHD4 chd4 (Catalog #AAA2542142) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CHD4 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHD4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:5000 IF: 1:50-1:500. Researchers should empirically determine the suitability of the CHD4 chd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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