Rabbit MANF Polyclonal Antibody | anti-MANF antibody

MANF Antibody

Cat. Num. AAA9403499

• Gene Names: MANF; ARP; ARMET
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: MANF; Polyclonal Antibody; MANF Antibody; ARP; ARMET; anti-MANF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total MANF protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 2.5 mg/ml (varies by lot)
• Sequence Length: 185

• Applicable Applications for anti-MANF antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:100-1:300
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to residues near the C terminal of human mesencephalic astrocyte-derived neurotrophic factor
• Target Name: MANF
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/70.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/70.)

Related Product Information for anti-MANF antibody

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of polymorphisms in the arginine-rich region, including a specific mutation that changes the previously numbered codon 50 from ATG to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus not tumor-related.

Product Categories/Family for anti-MANF antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 618857509
• NCBI GeneID: 7873
• NCBI Accession #: NP_006001.4
• NCBI GenBank Nucleotide #: NM_006010.5
• UniProt Accession #: P55145; Q14CX4; Q86U67; Q96IS4
• Molecular Weight: 20,700 Da
• NCBI Official Full Name: mesencephalic astrocyte-derived neurotrophic factor
• NCBI Official Synonym Full Names: mesencephalic astrocyte-derived neurotrophic factor
• NCBI Official Symbol: MANF
• NCBI Official Synonym Symbols: ARP; ARMET
• NCBI Protein Information: mesencephalic astrocyte-derived neurotrophic factor
• UniProt Protein Name: Mesencephalic astrocyte-derived neurotrophic factor
• Protein Family: MANF/CDNF-like protein
• UniProt Gene Name: MANF
• UniProt Synonym Gene Names: ARMET; ARP
• UniProt Entry Name: MANF_HUMAN

NCBI Description

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]

Uniprot Description

ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular space; endoplasmic reticulum; perinuclear region of cytoplasm; nucleus

Molecular Function: growth factor activity

Biological Process: vasoconstriction of artery involved in ischemic response to lowering of systemic arterial blood pressure; response to unfolded protein

Disease: Pancreatic Cancer