SCAP blocking peptide

SCAP Antibody (N-term) Blocking peptide

• Synonyms: SCAP; SCAP Antibody (N-term) Blocking peptide; Sterol regulatory element-binding protein cleavage-activating protein; SREBP cleavage-activating protein; KIAA0199; SCAP blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1279

• Cellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SCAP blocking peptide

Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 116242783
• NCBI GeneID: 22937
• UniProt Accession #: Q12770; Q8N2E0; Q8WUA1
• Molecular Weight: 95,616 Da
• NCBI Official Full Name: Sterol regulatory element-binding protein cleavage-activating protein
• NCBI Official Synonym Full Names: SREBF chaperone
• NCBI Official Symbol: SCAP
• NCBI Protein Information: sterol regulatory element-binding protein cleavage-activating protein
• UniProt Protein Name: Sterol regulatory element-binding protein cleavage-activating protein
• Protein Family: Sterol regulatory element-binding protein cleavage-activating protein
• UniProt Gene Name: SCAP
• UniProt Synonym Gene Names: KIAA0199; SCAP; SREBP cleavage-activating protein
• UniProt Entry Name: SCAP_HUMAN

NCBI Description

This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

SCAP: Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. Belongs to the WD repeat SCAP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: Golgi membrane

Molecular Function: protein binding

Biological Process: SREBP-mediated signaling pathway