Rabbit anti-Human, Mouse SCAP Polyclonal Antibody | anti-SCAP antibody

anti-SCAP antibody

Cat. Num. AAA7603855

• Gene Names: Scap; mKIAA0199; 9530044G19
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purification: Immunogen affinity purified; Purity: > = 95% as determined by SDS-PAGE
• Synonyms: SCAP; Polyclonal Antibody; anti-SCAP antibody; KIAA0199

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purification: Immunogen affinity purified; Purity: > = 95% as determined by SDS-PAGE
• Form/Format: Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3
• Sequence Length: 1276

• Applicable Applications for anti-SCAP antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
• Immunogen: SREBF chaperone
• Preparation and Storage: Store at -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using MBS7603855 (SCAP Antibody) at dilution of 1:50)

Western Blot (WB)

(HEK-293 cells were subjected to SDS PAGE followed by western blot with MBS7603855 (SCAP antibody) at dilution of 1:300)

Related Product Information for anti-SCAP antibody

Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway(By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 156938293
• NCBI GeneID: 235623
• NCBI Accession #: NP_001096632.1
• NCBI GenBank Nucleotide #: NM_001103162.2
• UniProt Accession #: Q12770; Q6A0A6; Q6NS67; Q7TNG7; Q80UI6; Q6GQT6
• Molecular Weight: Observed MW: 140 kDa,96 kDa
• NCBI Official Full Name: sterol regulatory element-binding protein cleavage-activating protein
• NCBI Official Synonym Full Names: SREBF chaperone
• NCBI Official Symbol: Scap
• NCBI Official Synonym Symbols: mKIAA0199; 9530044G19
• NCBI Protein Information: sterol regulatory element-binding protein cleavage-activating protein
• UniProt Protein Name: Sterol regulatory element-binding protein cleavage-activating protein
• Protein Family: Sterol regulatory element-binding protein cleavage-activating protein
• UniProt Gene Name: Scap
• UniProt Synonym Gene Names: SCAP; SREBP cleavage-activating protein
• UniProt Entry Name: SCAP_MOUSE

Uniprot Description

SCAP: Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. Belongs to the WD repeat SCAP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: cytoplasmic vesicle; endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; integral to membrane; intracellular membrane-bound organelle; membrane; protein complex

Molecular Function: cholesterol binding; protein binding; protein complex binding

Biological Process: cholesterol metabolic process; lipid metabolic process; regulation of cholesterol biosynthetic process; regulation of fatty acid biosynthetic process; regulation of fatty acid metabolic process; response to hypoxia; response to insulin stimulus; SREBP-mediated signaling pathway; steroid metabolic process