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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using RMI1 Antibody (MBS129174) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

Rabbit RMI1 Polyclonal Antibody | anti-RMI1 antibody

RMI1 Polyclonal Antibody

Gene Names
RMI1; BLAP75; FAAP75; C9orf76
Applications
Western Blot
Purity
Affinity Purification
Synonyms
RMI1; Polyclonal Antibody; RMI1 Polyclonal Antibody; BLAP75; FAAP75; C9orf76; anti-RMI1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
625
Applicable Applications for anti-RMI1 antibody
Western Blot (WB)
Application Notes
WB: 1:200 - 1:2000
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350of human RMI1 (NP_079221.2)
Species Reactivity/Cross Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human RMI1
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using RMI1 Antibody (MBS129174) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using RMI1 Antibody (MBS129174) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Related Product Information for anti-RMI1 antibody
RMI1 is a component of protein complexes that limit DNA crossover formation via the dissolution of double Holliday junctions (Raynard et al., 2006)
Product Categories/Family for anti-RMI1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed MW 75kDa
Calculated MW 70kDa
NCBI Official Full Name
RecQ-mediated genome instability protein 1
NCBI Official Synonym Full Names
RecQ mediated genome instability 1
NCBI Official Symbol
RMI1
NCBI Official Synonym Symbols
BLAP75; FAAP75; C9orf76
NCBI Protein Information
recQ-mediated genome instability protein 1; BLM-associated protein 75 kDa; BLM-associated polypeptide, 75 kDa; RMI1, RecQ mediated genome instability 1, homolog; homolog of yeast RecQ-mediated genome instability 1 (RMI1)
UniProt Protein Name
RecQ-mediated genome instability protein 1
UniProt Gene Name
RMI1
UniProt Synonym Gene Names
C9orf76; BLAP75
UniProt Entry Name
RMI1_HUMAN

NCBI Description

RMI1 is a component of protein complexes that limit DNA crossover formation via the dissolution of double Holliday junctions (Raynard et al., 2006 [PubMed 16595695]).[supplied by OMIM, Mar 2008]

Uniprot Description

RMI1: Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability. Belongs to the RMI1 family.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 9q21.32

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: multicellular organism growth; response to glucose stimulus; DNA replication; glucose homeostasis; reduction of food intake in response to dietary excess

Research Articles on RMI1

Similar Products

Product Notes

The RMI1 rmi1 (Catalog #AAA129174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RMI1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:200 - 1:2000. Researchers should empirically determine the suitability of the RMI1 rmi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RMI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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