Rat Akr7a2 (Aflatoxin B1 aldehyde reductase member 2) ELISA Kit | Akr7a2 elisa kit

Rat Akr7a2 (Aflatoxin B1 aldehyde reductase member 2) ELISA Kit

Cat. Num. AAA7607132

• Gene Names: AKR7A2; AFAR; AKR7; AFAR1; AFB1-AR1
• Reactivity: Rat
• Synonyms: Akr7a2 (Aflatoxin B1 aldehyde reductase member 2); Rat Akr7a2 (Aflatoxin B1 aldehyde reductase member 2) ELISA Kit; Akr7a2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Akr7a2. No significant cross-reactivity or interference between Akr7a2 and analogues was observed.
• Sequence Length: 324

• Samples: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: <46.875pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Akr7a2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Akr7a2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Akr7a2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Akr7a2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Akr7a2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Akr7a2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Akr7a2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 1004393738
• NCBI GeneID: 8574
• NCBI Accession #: NP_001307908.1
• NCBI GenBank Nucleotide #: NM_001320979.1
• Molecular Weight: 39,589 Da
• NCBI Official Full Name: aflatoxin B1 aldehyde reductase member 2 isoform 2
• NCBI Official Synonym Full Names: aldo-keto reductase family 7 member A2
• NCBI Official Symbol: AKR7A2
• NCBI Official Synonym Symbols: AFAR; AKR7; AFAR1; AFB1-AR1
• NCBI Protein Information: aflatoxin B1 aldehyde reductase member 2
• UniProt Protein Name: Aflatoxin B1 aldehyde reductase member 2
• Protein Family: Aflatoxin B1 aldehyde reductase
• UniProt Gene Name: AKR7A2
• UniProt Synonym Gene Names: AFAR; AFAR1; AKR7; AFB1-AR 1; SSA reductase

NCBI Description

The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.