Rabbit GIT1 Polyclonal Antibody | anti-GIT1 antibody

GIT1 (Y554) Antibody (C-term)

Cat. Num. AAA9217143

• Reactivity: Human, Mouse; Predicted Reactivity: Rat
• Applications: Western Blot, Flow Cytometry, Functional Assay, ELISA
• Synonyms: GIT1; Polyclonal Antibody; GIT1 (Y554) Antibody (C-term); ARF GTPase-activating protein GIT1; ARF GAP GIT1; Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1; anti-GIT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This GIT1-Y554 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 533-561 amino acids from the C-terminal region of human GIT1-Y554.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 761

• Applicable Applications for anti-GIT1 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: WB:~~1:1000; FC:~~1:10~50
• Subtitle: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Cytoplasm. Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.
• Antigen Region: 533-561 aa
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-Phospho-GIT1-pY554. ctrl at 1:2000 dilutionLane 1: U-2OS whole cell lysateLane 2: HUVEC whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(GIT1-Y554 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).)

Western Blot (WB)

(GIT1-Y554 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).)

Flow Cytometry (FC/FACS)

(GIT1-Y554 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GIT1 antibody

Description: GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes (from SwissProt).

Background: GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine- phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 (By similarity).

Product Categories/Family for anti-GIT1 antibody

Primary Antibodies; Signal Transduction

References

Hsu, R.M., et al. Mol. Biol. Cell 21 (2):287-301 (2010). Hajdo-Milasinovic, A., et al. J. Cell. Sci. 122 (PT 12), 2127-2136 (2009). Morimura, S., et al. Biochem. Biophys. Res. Commun. 382 (3):614-619 (2009). Wang, J., et al. Arterioscler. Thromb. Vasc. Biol. 29 (2):202-208 (2009). Zhan, L., et al. Cell 135 (5):865-878 (2008). Sugiyama, N., et al. Mol. Cell Proteomics 6 (6):1103-1109 (2007). Olsen, J.V., et al. Cell 127 (3):635-648 (2006). Webb, D.J., et al. J. Cell. Sci. 119 (PT 14), 2847-2850 (2006). Kim, J.E., et al. J. Proteome Res. 4 (4):1339-1346 (2005). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101 (33):12130-12135 (2004). Manabe, R., et al. J. Cell. Sci. 115 (PT 7), 1497-1510 (2002). Kawachi, H., et al. Proc. Natl. Acad. Sci. U.S.A. 98 (12):6593-6598 (2001). Zhao, Z.S., et al. Mol. Cell. Biol. 20 (17):6354-6363 (2000). Premont, R.T., et al. J. Biol. Chem. 275 (29):22373-22380 (2000). Premont, R.T., et al. Proc. Natl. Acad. Sci. U.S.A. 95 (24):14082-14087 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 41393573
• NCBI GeneID: 28964
• NCBI Accession #: NP_054749.2; NP_054749
• NCBI GenBank Nucleotide #: NM_014030.3
• UniProt Accession #: Q9Y2X7; Q86SS0; Q9BRJ4; B4DGU9; B4DSV3
• Molecular Weight: 84341 Da
• NCBI Official Full Name: ARF GTPase-activating protein GIT1 isoform 2
• NCBI Official Synonym Full Names: GIT ArfGAP 1
• NCBI Official Symbol: GIT1
• NCBI Protein Information: ARF GTPase-activating protein GIT1
• UniProt Protein Name: ARF GTPase-activating protein GIT1
• Protein Family: ARF GTPase-activating protein
• UniProt Gene Name: GIT1
• UniProt Synonym Gene Names: ARF GAP GIT1; CAT-1; CAT1
• UniProt Entry Name: GIT1_HUMAN

Uniprot Description

GIT1: a multidomain, adaptor protein that regulates cellular processes, such as migration and protrusive activity, by bringing together various signaling molecules, including PIX, PAK, and paxillin. Phosphorylation on Ser-709 increases its binding to paxillin and regulates cellular protrusions. Possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Functions as part of a multi-molecular signaling complex that regulates membrane protrusions and cell migration. Regulates PLC-gamma activation by c-Src and integrates signal transduction by G protein coupled receptors (GPCR)s and tyrosine kinase receptors (TKRs).

Protein type: Motility/polarity/chemotaxis; GAPs; GAPs, ARF

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cytoplasm; cytosol; focal adhesion; membrane

Molecular Function: GTPase activator activity; metal ion binding; protein binding; protein complex binding

Biological Process: ephrin receptor signaling pathway; positive regulation of GTPase activity; regulation of cytokinesis; regulation of G-protein coupled receptor protein signaling pathway