Western Blot (WB)
(All lanes : Anti-GIT1 Antibody at 1:1000 dilutionLane 1: U-2OS whole cell lysateLane 2: U-251 MG whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit GIT1-S388 Polyclonal Antibody | anti-GIT1 antibody
GIT1-S388 Antibody (Center)
Western Blot (WB)
(All lanes : Anti-GIT1 Antibody at 1:1000 dilutionLane 1: U-2OS whole cell lysateLane 2: U-251 MG whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GIT1 Antibody at 1:1000 dilutionLane 1: U-2OS whole cell lysateLane 2: U-251 MG whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(GIT1-S388 Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).)
Western Blot (WB)
(GIT1-S388 Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).)
Background: GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine- phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 (By similarity).
NCBI and Uniprot Product Information
Uniprot Description
GIT1: a multidomain, adaptor protein that regulates cellular processes, such as migration and protrusive activity, by bringing together various signaling molecules, including PIX, PAK, and paxillin. Phosphorylation on Ser-709 increases its binding to paxillin and regulates cellular protrusions. Possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Functions as part of a multi-molecular signaling complex that regulates membrane protrusions and cell migration. Regulates PLC-gamma activation by c-Src and integrates signal transduction by G protein coupled receptors (GPCR)s and tyrosine kinase receptors (TKRs).
Protein type: GAPs, ARF; GAPs; Motility/polarity/chemotaxis
Cellular Component: cytoplasm; focal adhesion; membrane; neuron projection; synapse
Molecular Function: GTPase activator activity; metal ion binding; protein binding; protein complex binding; protein homodimerization activity; small GTPase binding
Biological Process: ephrin receptor signaling pathway; formation of immunological synapse; positive regulation of GTPase activity; regulation of cytokinesis; regulation of G-protein coupled receptor protein signaling pathway
Research Articles on GIT1
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Product Notes
The GIT1 git1 (Catalog #AAA9217144) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GIT1-S388 Antibody (Center) reacts with Human, Mouse; Predicted Reactivity: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GIT1-S388 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:1000. Researchers should empirically determine the suitability of the GIT1 git1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GIT1-S388, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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