Western Blot (WB)
(ASAH2 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the ASAH2 antibody detected the ASAH2 protein (arrow).)
Rabbit anti-Human, mouse ASAH2 Polyclonal Antibody | anti-ASAH2 antibody
ASAH2 Antibody (C-term)
Western Blot (WB)
(ASAH2 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the ASAH2 antibody detected the ASAH2 protein (arrow).)
Western Blot (WB)
(ASAH2 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the ASAH2 antibody detected the ASAH2 protein (arrow).)
hydrolysis of the N-acyl linkage of ceramide, a second messenger in
a variety of cellular events, to produce sphingosine. Sphingosine
exerts both mitogenic and apoptosis-inducing activities, and its
phosphorylated form functions as an intra- and intercellular second
messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed
11328816]).
Hong, K.K., et al. J. Korean Med. Sci. 22(5):862-867(2007)
Ohlsson, L., et al. Biochimie 89(8):950-960(2007)
Avramopoulos, D., et al. Neurogenetics 8(2):111-120(2007)
Galadari, S., et al. Biochem. J. 393 (PT 3), 687-695 (2006) :
NCBI and Uniprot Product Information
NCBI Description
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]
Uniprot Description
ASAH2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Belongs to the neutral ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - sphingolipid; Membrane protein, integral; EC 3.5.1.23; Hydrolase
Chromosomal Location of Human Ortholog: 10q11.21
Cellular Component: mitochondrion; plasma membrane; integral to membrane
Molecular Function: ceramidase activity
Biological Process: sphingolipid metabolic process; apoptosis; glycosphingolipid metabolic process; signal transduction; ceramide metabolic process
Research Articles on ASAH2
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Product Notes
The ASAH2 asah2 (Catalog #AAA9210331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 734-762. The ASAH2 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ASAH2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the ASAH2 asah2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASAH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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