Western Blot (WB)
(PACRG Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PACRG antibody detected the PACRG protein (arrow).)
Rabbit anti-Human PACRG Polyclonal Antibody | anti-PACRG antibody
PACRG, NT (PACRG, GLUP, Parkin coregulated gene protein, Molecular chaperone/chaperonin-binding protein, PARK2 coregulated gene protein) (Azide free) (HRP)
Gene Names
PACRG; GLUP; PARK2CRG; HAK005771; RP3-495O10.2
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PACRG; Polyclonal Antibody; NT (PACRG; GLUP; Parkin coregulated gene protein; Molecular chaperone/chaperonin-binding protein; PARK2 coregulated gene protein) (Azide free) (HRP); PARK2 coregulated gene protein; anti-PACRG antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-PACRG antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
PACRG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-74 amino acids from the N-terminal region of human PACRG.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(PACRG Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PACRG antibody detected the PACRG protein (arrow).)
Western Blot (WB)
(PACRG Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PACRG antibody detected the PACRG protein (arrow).)
Related Product Information for anti-PACRG antibody
This gene encodes a protein that is conserved across metazoans. In vertebrates, this gene is linked in a head-to-head arrangement with the adjacent parkin gene, which is associated with autosomal recessive juvenile Parkinson's disease. These genes are co-regulated in various tissues and they share a bi-directional promoter. Both genes are associated with susceptibility to leprosy. The parkin co-regulated gene protein forms a large molecular complex with chaperones, including heat shock proteins 70 and 90, and chaperonin components. This protein is also a component of Lewy bodies in Parkinson's disease patients, and it suppresses unfolded Pael receptor-induced neuronal cell death. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-PACRG antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Wilson, G.R., et al. Fertil. Steril. 93(7):2262-2268(2010). Dagda, R.K., et al. J. Bioenerg. Biomembr. 41(6):473-479(2009). Velez, D.R., et al. Int. J. Tuberc. Lung Dis. 13(9):1068-1076(2009). Taylor, J.M., et al. Parkinsonism Relat. Disord. 15(6):417-421(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,342 Da
NCBI Official Full Name
parkin coregulated gene protein isoform 2
NCBI Official Synonym Full Names
PARK2 co-regulated
NCBI Official Symbol
PACRG
NCBI Official Synonym Symbols
GLUP; PARK2CRG; HAK005771; RP3-495O10.2
NCBI Protein Information
parkin coregulated gene protein; parkin co-regulated gene protein; molecular chaperone/chaperonin-binding protein
UniProt Protein Name
Parkin coregulated gene protein
Protein Family
UniProt Gene Name
PACRG
UniProt Synonym Gene Names
GLUP
UniProt Entry Name
PACRG_HUMAN
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Product Notes
The PACRG pacrg (Catalog #AAA6330410) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PACRG, NT (PACRG, GLUP, Parkin coregulated gene protein, Molecular chaperone/chaperonin-binding protein, PARK2 coregulated gene protein) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PACRG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PACRG pacrg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PACRG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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