Mouse anti-Human EIF4E2 Monoclonal Antibody | anti-EIF4E2 antibody

EIF4E2 (EIF4E2, EIF4EL3, Eukaryotic translation initiation factor 4E type 2, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E-like 3, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, mRNA

Cat. Num. AAA6120821

• Gene Names: EIF4E2; 4EHP; IF4e; 4E-LP; h4EHP; EIF4EL3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: EIF4E2; Monoclonal Antibody; EIF4E2 (EIF4E2; EIF4EL3; Eukaryotic translation initiation factor 4E type 2; Eukaryotic translation initiation factor 4E homologous protein; Eukaryotic translation initiation factor 4E-like 3; eIF4E-like protein 4E-LP; mRNA cap-binding protein 4EHP; mRNA; mRNA cap-binding protein type 3; anti-EIF4E2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 225CT3.1.3
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-EIF4E2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1000; WB: 1:100-250; Applications are based on unconjugated antibody.
• Immunogen: EIF4E2 Mab is generated from mouse immunized with EIF4E2 recombinant protein.
• Conjugate: AP
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C with the addition of glycerol. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive.

Western Blot (WB)

(EIF4E2/MB10227 antibody western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the EIF4E2/MB10227 antibody detected the EIF4E2/MB10227 protein (arrow).)

Related Product Information for anti-EIF4E2 antibody

EIF4E2 recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Product Categories/Family for anti-EIF4E2 antibody

Antibodies; Translation Initiation Factors

References

Rose, J. Phd, et al. Mol. Med. (2010) In press Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009) Rosettani, P., et al. J. Mol. Biol. 368(3):691-705(2007) Zuberek, J., et al. RNA 13(5):691-697(2007) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)

NCBI and Uniprot Product Information

• NCBI GI #: 4757702
• NCBI GeneID: 9470
• NCBI Accession #: NP_004837.1
• NCBI GenBank Nucleotide #: NM_004846.3
• UniProt Accession #: O60573
• NCBI Official Full Name: eukaryotic translation initiation factor 4E type 2 isoform A
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E family member 2
• NCBI Official Symbol: EIF4E2
• NCBI Official Synonym Symbols: 4EHP; IF4e; 4E-LP; h4EHP; EIF4EL3
• NCBI Protein Information: eukaryotic translation initiation factor 4E type 2
• UniProt Protein Name: Eukaryotic translation initiation factor 4E type 2
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF4E2
• UniProt Synonym Gene Names: EIF4EL3; eIF-4E type 2; eIF4E type 2
• UniProt Entry Name: IF4E2_HUMAN

Uniprot Description

EIF4E2: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E and eIF4G. eIF4E is also known to interact with other partners. EIF4E2 interacts with EIF4EBP1, EIF4EBP2 and EIF4EBP3 but not with eIF4G. Belongs to the eukaryotic initiation factor 4E family.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: mRNA cap complex; cytosol

Molecular Function: protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity; ubiquitin protein ligase binding; RNA cap binding

Biological Process: in utero embryonic development; cytokine and chemokine mediated signaling pathway; negative regulation of translation; translational initiation