Rabbit anti-Human, Mouse ASAH2 Polyclonal Antibody | anti-ASAH2 antibody

ASAH2, CT (ASAH2, HNAC1, Neutral ceramidase, Acylsphingosine deacylase 2, BCDase, LCDase, N-acylsphingosine amidohydrolase 2, Non-lysosomal ceramidase, Neutral ceramidase soluble form) (MaxLight 550)

Cat. Num. AAA6275475

• Gene Names: ASAH2B; ASAH2C; ASAH2L; bA98I6.3; bA449O16.3
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ASAH2; Polyclonal Antibody; CT (ASAH2; HNAC1; Neutral ceramidase; Acylsphingosine deacylase 2; BCDase; LCDase; N-acylsphingosine amidohydrolase 2; Non-lysosomal ceramidase; Neutral ceramidase soluble form) (MaxLight 550); Neutral ceramidase soluble form; anti-ASAH2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ASAH2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-ASAH2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 734-762 in the C-terminal region of human ASAH2, UniProt Accession #NP_001072984.1; Q9NR71; P0C7U2; P0C7U1.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse bladder tissue lysates (35ug/lane) usingMBS6005122. This demonstrates that MBS6005122 detected the ASAH2 protein (arrow).)

Related Product Information for anti-ASAH2 antibody

Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra-and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).

Product Categories/Family for anti-ASAH2 antibody

Antibodies; Enzymes

References

1. Uchida, Y., et al. J. Invest. Dermatol. 130(10):2472-2480(2010). 2. Hong, K.K., et al. J. Korean Med. Sci. 22(5):862-867(2007). 3. Ohlsson, L., et al. Biochimie 89(8):950-960(2007). 4. Avramopoulos, D., et al. Neurogenetics 8(2):111-120(2007). 5. Galadari, S., et al. Biochem. J. 393 (PT 3), 687-695 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 118442845
• NCBI GeneID: 653308
• NCBI Accession #: NP_001072984.1
• NCBI GenBank Nucleotide #: NM_001079516.3
• UniProt Accession #: Q9NR71; P0C7U2; P0C7U1
• Molecular Weight: 18,026 Da
• NCBI Official Full Name: putative inactive neutral ceramidase B isoform 1
• NCBI Official Synonym Full Names: N-acylsphingosine amidohydrolase 2B
• NCBI Official Symbol: ASAH2B
• NCBI Official Synonym Symbols: ASAH2C; ASAH2L; bA98I6.3; bA449O16.3
• NCBI Protein Information: putative inactive neutral ceramidase B
• UniProt Protein Name: Neutral ceramidase
• Protein Family: Neutral ceramidase
• UniProt Gene Name: ASAH2
• UniProt Synonym Gene Names: HNAC1; N-CDase; NCDase; hCD
• UniProt Entry Name: ASAH2_HUMAN

Uniprot Description

ASAH2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Belongs to the neutral ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Hydrolase; EC 3.5.1.23; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 10q11.21

Cellular Component: mitochondrion; plasma membrane; integral to membrane

Molecular Function: ceramidase activity

Biological Process: sphingolipid metabolic process; apoptosis; glycosphingolipid metabolic process; ceramide metabolic process; signal transduction